School of Biological Science and Technology, Central South University, Changsha 410013, China.
Anal Biochem. 2012 Oct 1;429(1):8-10. doi: 10.1016/j.ab.2012.06.022. Epub 2012 Jul 1.
A novel fluorescent probe for adenosine triphosphate (ATP) assay based on DNA ligation is proposed in this article. This approach uses a novel smart probe, T4 DNA ligase, and two short oligonucleotides. In the presence of ATP, the T4 DNA ligase catalyzes the ligation reaction and the ligation product restores the fluorescence of the smart probe. This method is very sensitive with a 0.5-nM limit of detection. Compared with current assay methods, the strategy is simpler, cheaper, and 40 times more sensitive.
本文提出了一种基于 DNA 连接的新型用于三磷酸腺苷（ATP）检测的荧光探针。该方法使用了一种新型的智能探针 T4 DNA 连接酶和两个短的寡核苷酸。在存在 ATP 的情况下，T4 DNA 连接酶催化连接反应，连接产物恢复智能探针的荧光。该方法具有 0.5 nM 的检测限，非常灵敏。与目前的检测方法相比，该策略更简单、更便宜，灵敏度提高了 40 倍。
