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用锥形管自然沉淀法进行寄生虫学粪便样本检查:效果、实践和生物安全。

Parasitological stool sample exam by spontaneous sedimentation method using conical tubes: effectiveness, practice, and biosafety.

机构信息

Núcleo de Doenças Infecciosas, Universidade Federal de Espírito Santo, Vitória, ES, Brasil.

出版信息

Rev Soc Bras Med Trop. 2012 Jun;45(3):399-401. doi: 10.1590/s0037-86822012000300024.

Abstract

INTRODUCTION

Spontaneous sedimentation is an important procedure for stool examination. A modification of this technique using conical tubes was performed and evaluated.

METHODS

Fifty fecal samples were processed in sedimentation glass and in polypropylene conical tubes. Another 50 samples were used for quantitative evaluation of protozoan cysts.

RESULTS

Although no significant differences occurred in the frequency of protozoa and helminths detected, significant differences in protozoan cyst counts did occur.

CONCLUSIONS

The use of tube predicts a shorter path in the sedimentation of the sample, increases concentration of parasites for microscopy analysis, minimizes the risks of contamination, reduces the odor, and optimizes the workspace.

摘要

简介

自然沉降是粪便检查的重要程序。我们对该技术进行了改良,使用了锥形管,并对其进行了评估。

方法

50 份粪便样本分别用沉降玻璃管和聚丙烯锥形管进行处理。另外 50 份样本用于对原虫包囊进行定量评估。

结果

虽然检测到的原虫和寄生虫的频率没有显著差异,但原虫包囊的数量确实存在显著差异。

结论

使用锥形管可以预测样本沉降的路径更短,增加寄生虫的浓度以便进行显微镜分析,最大限度地减少污染风险,减少气味,并优化工作空间。

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