Tompa K, Bokor M, Tompa P
Research Institute for Solid State Physics and Optics, Hungarian Academy of Sciences, Budapest, Hungary.
Methods Mol Biol. 2012;895:167-96. doi: 10.1007/978-1-61779-927-3_13.
In this chapter, the reader is introduced to the basics of wide-line NMR, with particular focus on the following: (1) basic theoretical and experimental NMR elements, necessary before switching the spectrometer and designing the experiment, (2) models/theories for the interpretation of measured data, (3) definition of wide-line NMR spectrometry, the description of the measurement and evaluation variants, useful hints for the novice, (4) advice on selecting the solvent, which is not a trivial task, (5) a note of warning that not all data are acceptable in spite of the statistical confidence. Finally, we wrap up the chapter with the results on two proteins (a globular and an intrinsically disordered).
在本章中，向读者介绍宽线核磁共振的基础知识，特别关注以下内容：（1）在开启光谱仪和设计实验之前所需的基本理论和实验核磁共振元素；（2）用于解释测量数据的模型/理论；（3）宽线核磁共振光谱法的定义、测量和评估变体的描述、给新手的有用提示；（4）关于选择溶剂的建议，这并非易事；（5）一则警告，即尽管有统计置信度，但并非所有数据都是可接受的。最后，我们以两种蛋白质（一种球状蛋白质和一种内在无序蛋白质）的结果结束本章。
