紫外可见吸收光谱法在天然无序蛋白质研究中的应用。
The use of UV-Vis absorption spectroscopy for studies of natively disordered proteins.
作者信息
Permyakov Eugene A
机构信息
Institute for Biological Instrumentation of the Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
出版信息
Methods Mol Biol. 2012;895:421-33. doi: 10.1007/978-1-61779-927-3_24.
PMID:22760331
Abstract
Absorption spectroscopy can be used to monitor structural changes upon transitions from ordered to disordered state in proteins. Changes in environment of tryptophan, tyrosine, and phenylalanine residues result in changes of their absorption spectra. In most cases the changes are small and can be measured only in a differential mode.
摘要
吸收光谱可用于监测蛋白质从有序状态转变为无序状态时的结构变化。色氨酸、酪氨酸和苯丙氨酸残基环境的变化会导致其吸收光谱的改变。在大多数情况下，这些变化很小，只能以差分模式进行测量。
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