Bhatt Priya, Garad Prajakta Sharad, Rayala V V S Prasanna Kumari, Radhakrishnanand P, Sankaranarayanan Kamatchi
Physical Sciences Division, Institute of Advanced Study in Science and Technology, (An Autonomous Institute Under DST, Govt. of India) Vigyan Path, Paschim Boragaon, Garchuk Guwahati Assam 781035 India
Academy of Scientific and Innovative Research (AcSIR), Campus Postal Staff College Area Sector 19, Kamla Nehru Nagar Ghaziabad 201002 Uttar Pradesh India.
RSC Adv. 2024 Apr 29;14(20):13984-13996. doi: 10.1039/d4ra01891e. eCollection 2024 Apr 25.
Aromatic amino acids (AAs) have garnered particular interest due to their pivotal roles in numerous biological processes and disorders. Variations in AA self-assembly not only affect protein structures and functions, but their non-covalent interactions such as hydrogen bonding, van der Waals forces, and π-π stacking, yield versatile assemblies vital in bio-inspired material fabrication. Tyrosine (Tyr), a non-essential aromatic amino acid, holds multifaceted significance in the body as a protein building block, neurotransmitter precursor, thyroid hormone contributor, and melanin synthesis regulator. The proficiency of Cold Atmospheric Plasma (CAP) in generating a spectrum of reactive oxygen and nitrogen species has spurred innovative research avenues in the studies of biomolecular components, including its potential for targeted cancer cell ablation and biomolecule modification. In this work, we have assessed the chemical as well as the structural changes in Tyrosine-derived self-assembled structures arising from the CAP-induced reactive species. For a comprehensive understanding of the mechanism, different treatment times, feed gases, and the role of solvent acidification are compared using various spectroscopic and microscopic techniques. LC-ESI-QQQ mass spectra unveiled the emergence of oxygenated and nitro derivatives of l-tyrosine following its interaction with CAP-derived ROS/RNS. SEM and TEM images demonstrated an enhanced surface size of self-assembled structures and the formation of novel nanomaterial-shaped assemblies following CAP treatment. Overall, this study aims to explore CAP's interaction with a single-amino acid, hypothesizing the creation of novel supramolecular structures and scrutinizing CAP-instigated transformations in l-tyrosine self-assembled structures, potentially advancing biomimetic-attributed nanomaterial fabrication which might present a novel frontier in the field of designing functional biomaterials.
芳香族氨基酸（AAs）因其在众多生物过程和疾病中发挥的关键作用而备受关注。氨基酸自组装的变化不仅会影响蛋白质的结构和功能，而且其非共价相互作用，如氢键、范德华力和π-π堆积，会产生对生物启发材料制造至关重要的多功能组装体。酪氨酸（Tyr）是一种非必需的芳香族氨基酸，在人体中具有多方面的重要意义，它是蛋白质的组成部分、神经递质前体、甲状腺激素的贡献者以及黑色素合成调节剂。冷大气等离子体（CAP）产生一系列活性氧和氮物种的能力，在生物分子成分的研究中开辟了创新的研究途径，包括其在靶向癌细胞消融和生物分子修饰方面的潜力。在这项工作中，我们评估了CAP诱导的活性物种对酪氨酸衍生的自组装结构产生的化学和结构变化。为了全面了解其机制，我们使用各种光谱和显微镜技术比较了不同的处理时间、进料气体以及溶剂酸化的作用。液相色谱 - 电喷雾 - 三重四极杆质谱（LC - ESI - QQQ）揭示了L - 酪氨酸与CAP衍生的活性氧/氮物种相互作用后，出现了其氧化和硝基衍生物。扫描电子显微镜（SEM）和透射电子显微镜（TEM）图像显示，CAP处理后自组装结构的表面尺寸增大，并且形成了新型的纳米材料形状的组装体。总体而言，本研究旨在探索CAP与单一氨基酸的相互作用，推测新型超分子结构的形成，并仔细研究CAP引发的L - 酪氨酸自组装结构的转变，这可能推动仿生纳米材料的制造，为功能性生物材料设计领域带来新的前沿进展。
