Dipartimento di Scienze del Farmaco, Università 'G. D'Annunzio' Chieti-Pescara, Via dei Vestini 31, 66100, Chieti, Italy.
Phytother Res. 2013 May;27(5):784-6. doi: 10.1002/ptr.4773. Epub 2012 Jul 4.
Recent studies indicate that auraptene (7-geranyloxycoumarin, AUR), a geranyloxycoumarin extracted from fruits of edible plants belonging to the Rutaceae family, may represent a novel lead compound for dietary colon cancer chemoprevention in rodents. As a continuation of studies aimed to better depict the pharmacological effects and mechanism of action of the title natural compound, the current investigation was undertaken to determine whether AUR would be able to prevent the growth and sphere (surrogate tumors) formation of FOLFOX-resistant colon cancer cells that are highly enriched in cancer stem cells (CSCs). Our results demonstrate that AUR at a concentration of 10 μM was able to inhibit the growth and formation of colonospheres of FOLFOX-resistant colon cancer HT-29 cells in vitro. The corresponding parental cells were also similarly affected by AUR at the same concentration level. The reduction in the growth and colonospheres formation in FOLFOX-resistant HT-29 was also associated with a concomitant decrease in phospho-epidermal growth factor receptor. These findings suggest that AUR could prevent the re-emergence of CSCs.
最近的研究表明，从芸香科植物果实中提取的一种香叶基香豆素——auraptene（7-香叶基香豆素，AUR），可能是一种新型的用于预防啮齿动物结肠癌的饮食化学预防先导化合物。作为旨在更好地描述标题天然化合物的药理作用和作用机制的研究的延续，本研究旨在确定 AUR 是否能够预防富含癌症干细胞（CSCs）的 FOLFOX 耐药结肠癌细胞的生长和球体（替代肿瘤）形成。我们的研究结果表明，浓度为 10 μM 的 AUR 能够抑制体外 FOLFOX 耐药结肠癌细胞 HT-29 的生长和结肠球体形成。相同浓度水平的相应亲本细胞也受到 AUR 的类似影响。FOLFOX 耐药 HT-29 中生长和结肠球体形成的减少也与磷酸化表皮生长因子受体的同时减少相关。这些发现表明 AUR 可以防止 CSCs 的再次出现。
