Carter S R, Williams J M, Clamp J R
Chemistry Department, University College of Swansea, Great Britain.
Carbohydr Res. 1990 Sep 19;205:181-90. doi: 10.1016/0008-6215(90)80138-s.
Analysis of the oligosaccharides released from pig gastric mucus glycopolypeptides by hydrazinolysis showed that degradation had occurred. Nitrosation of the products followed by reduction gave a mixture that had a low content of 2,5-anhydro-D-talitol, which implied destruction of much of the terminal reducing 2-amino-2-deoxy-D-galactose. Under the conditions of hydrazinolysis, cellobiose was largely unchanged but laminaribiose gave a complex mixture that probably contained glucose hydrazone (13C-n.m.r. data). In order to avoid degradation, the hydrazinolysis-nitrosation sequence should be applied to the reduced oligosaccharides released on cleavage with alkali.
对经肼解从猪胃黏液糖多肽释放出的寡糖进行分析表明发生了降解。产物经亚硝化后再还原，得到的混合物中2,5-脱水-D-木糖醇含量较低，这意味着大部分末端还原性2-氨基-2-脱氧-D-半乳糖已被破坏。在肼解条件下，纤维二糖基本未变，但昆布二糖产生了一个可能含有葡萄糖腙的复杂混合物（碳-13核磁共振数据）。为避免降解，肼解-亚硝化序列应应用于用碱裂解释放出的还原寡糖。
