Krotkiewski H, Lisowska E
Arch Immunol Ther Exp (Warsz). 1978;26(1-6):139-44.
Three alkali-stable oligosaccharides free of amino acids were purified from hydrazinolysis products of M or N blood group glycoprotein. These three oligosaccharides differed in the content of sialic acid and contained similar amounts of other carbohydrate components. The asparagine-linked oligosaccharides, obtained by strong alkaline degradation of M glycoprotein, showed similar electrophoretic mobilities and chemical composition to the oligosaccharides obtained by hydrazinolysis. It indicates that hydrazinolysis does not give a substantial degradation of carbohydrates of alkali-stable oligosaccharides of human red cell glycoprotein.
从M或N血型糖蛋白的肼解产物中纯化出三种不含氨基酸的碱稳定寡糖。这三种寡糖的唾液酸含量不同，但其他碳水化合物成分的含量相似。通过对M糖蛋白进行强碱降解得到的天冬酰胺连接寡糖，其电泳迁移率和化学组成与通过肼解得到的寡糖相似。这表明肼解不会使人类红细胞糖蛋白的碱稳定寡糖的碳水化合物发生大量降解。
