Department of Respiratory Medicine, Nantong University Affiliated Hospital, Nantong, Jiangsu, China.
PLoS One. 2012;7(6):e39937. doi: 10.1371/journal.pone.0039937. Epub 2012 Jun 28.
We determined the expression of forkhead box Q1 (FoxQ1), E-cadherin (E-cad), Mucin 1 (MUC1), vimentin (VIM) and S100 calcium binding protein A4 (S100A4), all epithelial-mesenchymal transition (EMT) indicator proteins in non-small cell lung cancer (NSCLC) tissue samples. We also investigated the relationship between these five proteins expression and other clinicopathologic factors in NSCLC. Finally, we assessed the potential value of these markers as prognostic indicators of survival in NSCLC's patients.
Quantitative real-time PCR and immunohistochemistry were used to characterize the expression of the FoxQ1 mRNA and protein in NSCLC. Expression of transcripts and translated products for the other four EMT indicator proteins was assessed by immunohistochemistry in the same clinical NSCLC samples.
FoxQ1 mRNA and protein were up-regulated in NSCLC compared with normal tissues (P = 0.015 and P<0.001, respectively). Expression of FoxQ1 in adenocarcinoma was higher than in squamous cell carcinoma (P = 0.005), and high expression of FoxQ1 correlated with loss of E-cad expression (P = 0.012), and anomalous positivity of VIM (P = 0.024) and S100A4 (P = 0.004). Additional survival analysis showed that high expression of FoxQ1 (P = 0.047) and E-cad (P = 0.021) were independent prognostic factors.
FoxQ1 maybe plays a specific role in the EMT of NSCLC, and could be used as a prognostic factor for NSCLC.
我们测定了叉头框 Q1（FoxQ1）、E-钙黏蛋白（E-cad）、黏蛋白 1（MUC1）、波形蛋白（VIM）和 S100 钙结合蛋白 A4（S100A4）在非小细胞肺癌（NSCLC）组织样本中的表达，这些都是上皮间质转化（EMT）的指标蛋白。我们还研究了这 5 种蛋白表达与 NSCLC 其他临床病理因素之间的关系。最后，我们评估了这些标志物作为 NSCLC 患者生存预后指标的潜在价值。
使用定量实时 PCR 和免疫组织化学法检测 NSCLC 中 FoxQ1 mRNA 和蛋白的表达。在相同的临床 NSCLC 样本中，通过免疫组织化学法评估其他 4 种 EMT 指标蛋白的转录本和翻译产物的表达。
FoxQ1mRNA 和蛋白在 NSCLC 中均呈上调表达，与正常组织相比（P = 0.015 和 P<0.001）。FoxQ1 在腺癌中的表达高于鳞癌（P = 0.005），FoxQ1 高表达与 E-cad 表达缺失（P = 0.012），以及 VIM（P = 0.024）和 S100A4 （P = 0.004）异常阳性相关。进一步的生存分析表明，FoxQ1（P = 0.047）和 E-cad（P = 0.021）高表达是独立的预后因素。
FoxQ1 可能在 NSCLC 的 EMT 中发挥特定作用，可作为 NSCLC 的预后因素。
