Institute of Molecular Biology, Academia Sinica, Nankang, Taipei, 115 Taiwan.
Biosci Rep. 2012 Aug;32(4):345-59. doi: 10.1042/BSR20120007.
RNA splicing is one of the fundamental processes in gene expression in eukaryotes. Splicing of pre-mRNA is catalysed by a large ribonucleoprotein complex called the spliceosome, which consists of five small nuclear RNAs and numerous protein factors. The spliceosome is a highly dynamic structure, assembled by sequential binding and release of the small nuclear RNAs and protein factors. DExD/H-box RNA helicases are required to mediate structural changes in the spliceosome at various steps in the assembly pathway and have also been implicated in the fidelity control of the splicing reaction. Other proteins also play key roles in mediating the progression of the spliceosome pathway. In this review, we discuss the functional roles of the protein factors involved in the spliceosome pathway primarily from studies in the yeast system.
RNA 剪接是真核生物基因表达的基本过程之一。前体 mRNA 的剪接由一个称为剪接体的大型核糖核蛋白复合物催化，该复合物由五个小核 RNA 和众多蛋白质因子组成。剪接体是一种高度动态的结构，通过小核 RNA 和蛋白质因子的顺序结合和释放来组装。DExD/H 框 RNA 解旋酶需要介导剪接体在组装途径的各个步骤中的结构变化，并且还与剪接反应的保真度控制有关。其他蛋白质也在介导剪接体途径的进展中发挥关键作用。在这篇综述中，我们主要从酵母系统的研究中讨论了参与剪接体途径的蛋白质因子的功能作用。
