Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA.
RNA. 2011 Apr;17(4):551-4. doi: 10.1261/rna.2577511. Epub 2011 Feb 28.
Two recent papers, one from the Staley laboratory (Koodathingal and colleagues) and the other from the Cheng laboratory (Tseng and colleagues), show that the RNA-dependent ATPase Prp16, which is required for the second step of splicing, acts to reject slowly splicing pre-mRNAs immediately before the first catalytic reaction in pre-mRNA splicing. The results answer long-investigated questions about the actions of Prp16 and provide a wealth of molecular details on the proofreading process in pre-mRNA splicing. The discussion here reviews and integrates the results of the two papers and describes the implications for proofreading in splicing.
两篇最近的论文，一篇来自 Staley 实验室（Koodathingal 和同事），另一篇来自 Cheng 实验室（Tseng 和同事），表明 RNA 依赖性 ATP 酶 Prp16 是剪接第二步所必需的，它作用于在剪接前体 RNA 的第一个催化反应之前立即拒绝缓慢剪接的前体 RNA。这些结果解答了长期以来关于 Prp16 作用的问题，并提供了剪接中校对过程的丰富分子细节。这里的讨论回顾并整合了这两篇论文的结果，并描述了它们对剪接中校对的意义。
