剪接正确：前体 mRNA 剪接保真度的保障者。
The splice is right: guarantors of fidelity in pre-mRNA splicing.
机构信息
Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA.
出版信息
RNA. 2011 Apr;17(4):551-4. doi: 10.1261/rna.2577511. Epub 2011 Feb 28.
Abstract
Two recent papers, one from the Staley laboratory (Koodathingal and colleagues) and the other from the Cheng laboratory (Tseng and colleagues), show that the RNA-dependent ATPase Prp16, which is required for the second step of splicing, acts to reject slowly splicing pre-mRNAs immediately before the first catalytic reaction in pre-mRNA splicing. The results answer long-investigated questions about the actions of Prp16 and provide a wealth of molecular details on the proofreading process in pre-mRNA splicing. The discussion here reviews and integrates the results of the two papers and describes the implications for proofreading in splicing.
摘要
两篇最近的论文，一篇来自 Staley 实验室（Koodathingal 和同事），另一篇来自 Cheng 实验室（Tseng 和同事），表明 RNA 依赖性 ATP 酶 Prp16 是剪接第二步所必需的，它作用于在剪接前体 RNA 的第一个催化反应之前立即拒绝缓慢剪接的前体 RNA。这些结果解答了长期以来关于 Prp16 作用的问题，并提供了剪接中校对过程的丰富分子细节。这里的讨论回顾并整合了这两篇论文的结果，并描述了它们对剪接中校对的意义。
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本文引用的文献
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RNA. 2011 Jan;17(1):145-54. doi: 10.1261/rna.2459611. Epub 2010 Nov 22.
2
The DEAH box ATPases Prp16 and Prp43 cooperate to proofread 5' splice site cleavage during pre-mRNA splicing.DEAH 盒 ATP 酶 Prp16 和 Prp43 合作在 pre-mRNA 剪接过程中校对 5' 剪接位点切割。
Mol Cell. 2010 Aug 13;39(3):385-95. doi: 10.1016/j.molcel.2010.07.014.
3
Spliceosome discards intermediates via the DEAH box ATPase Prp43p.剪接体通过DEAH盒ATP酶Prp43p丢弃中间体。
Proc Natl Acad Sci U S A. 2010 Jun 1;107(22):10020-5. doi: 10.1073/pnas.0906022107. Epub 2010 May 12.
4
Release of SF3 from the intron branchpoint activates the first step of pre-mRNA splicing.内含子分支点处 SF3 的释放激活了前体 mRNA 剪接的第一步。
RNA. 2010 Mar;16(3):516-28. doi: 10.1261/rna.2030510. Epub 2010 Jan 20.
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The evolutionarily conserved core design of the catalytic activation step of the yeast spliceosome.酵母剪接体催化激活步骤的进化保守核心设计。
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