Jian Yuekui, Tian Xiaobin, Li Qing, Li Bo, Peng Zhi
Department of Orthopaedics, Guizhou Provincial People's Hospital, Guiyang, China.
Biotech Histochem. 2012 Aug;87(6):428-31. doi: 10.3109/10520295.2012.696701. Epub 2012 Jul 5.
Detection of microvessels is critical for studying bone tissue. We developed an intravascular ink-based method coupled with Van Gieson (VG) staining and compared it with other commonly used methods for capillary visualization. The ink perfusate was formulated as 10% ink, 10% formaldehyde and 20% mannitol. The ink solution was perfused into a healthy goat and the tibia was subjected to decalcification, dehydration, paraffin embedding, de-waxing and staining to observe microvessels. Angiogenesis was assessed by vascular area image analysis and the hematoxylin and eosin (HE), Masson, and VG staining techniques were compared to determine the reliability of these methods for counting microvessels. We found that HE, Masson, and VG staining produced poor contrast between the microvessels and surrounding tissues. By contrast, ink coupled with VG staining permitted clear discrimination between the microvessels and surrounding tissues. Our results indicate that ink-VG staining could be more useful than other methods for detecting tissue microvessels.
微血管的检测对于骨组织研究至关重要。我们开发了一种基于血管内墨水并结合范吉森（VG）染色的方法，并将其与其他常用的毛细血管可视化方法进行比较。墨水灌注液的配方为10%墨水、10%甲醛和20%甘露醇。将墨水溶液灌注到一只健康山羊体内，然后对胫骨进行脱钙、脱水、石蜡包埋、脱蜡和染色以观察微血管。通过血管面积图像分析评估血管生成，并比较苏木精和伊红（HE）、马松和VG染色技术，以确定这些方法在计数微血管方面的可靠性。我们发现，HE、马松和VG染色在微血管与周围组织之间产生的对比度较差。相比之下，墨水结合VG染色能够清晰地区分微血管与周围组织。我们的结果表明，墨水-VG染色在检测组织微血管方面可能比其他方法更有用。
