尿液对草酸钙“体外”结晶速率的影响：采用[¹⁴C]草酸盐技术测定抑制活性。

Influence of urine on "in vitro" crystallization rate of calcium oxalate: determination of inhibitory activity by a [14C]oxalate technique.

作者信息

Ligabue A, Fini M, Robertson W G

出版信息

Clin Chim Acta. 1979 Oct 15;98(1-2):39-46. doi: 10.1016/0009-8981(79)90163-3.

Abstract

A simple radiochemical method is proposed for the in vitro assay of the inhibitory activity of urine with respect to calcium oxalate crystal growth using [14C]oxalate as a tracer. The method shows an improved sensitivity over existing methods and indicates that citrate, pyrophosphate and chondroitin sulphate are active inhibitors of calcium oxalate crystal growth down to concentrations of 10(-5), 10(-7) and 10(-10) mol/l respectively. The inhibitory activity in the urines of 12 recurrent calcium stone-formers was significantly lower than in the urines of matched control subjects (P less than 0.01), confirming the clinical usefulness of the test.

摘要

提出了一种简单的放射化学方法，以[¹⁴C]草酸盐为示踪剂，用于体外测定尿液对草酸钙晶体生长的抑制活性。该方法比现有方法具有更高的灵敏度，表明柠檬酸盐、焦磷酸盐和硫酸软骨素分别在低至10⁻⁵、10⁻⁷和10⁻¹⁰mol/L的浓度下是草酸钙晶体生长的有效抑制剂。12名复发性草酸钙结石患者尿液中的抑制活性明显低于匹配的对照受试者尿液中的抑制活性（P<0.01），证实了该检测方法的临床实用性。