College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, People's Republic of China.
Carbohydr Polym. 2013 Jun 5;95(1):64-71. doi: 10.1016/j.carbpol.2013.02.033. Epub 2013 Feb 26.
In this study, a novel sulfated derivative of chitosan, which could be dissolved in many common organic solvents, is conveniently synthesized for the modification of polyethersulfone (PES) membrane. Elemental analysis, FTIR, (1)H NMR and X-ray diffraction diagrams (XRD) are used to demonstrate the introduction of functional groups. Owing to the solubility in organic solvents, the sulfated derivative of chitosan could be directly blended with PES in organic solvent to prepare membrane by means of a liquid-liquid phase separation technique. The modified membrane showed lower protein (bovine serum albumin (BSA) and bovine serum fibrinogen (BFG)) adsorption and suppressed platelet adhesion. Moreover, the activated partial thromboplastin time (APTT) for the modified membrane was enhanced as high as 60% compared to pure PES membrane. The lower protein adsorption, suppressed platelet adhesion and increased APTT confirmed that the blood compatibility of the modified PES membrane by the sulfated derivative of chitosan was significantly improved.
在这项研究中，合成了一种新型的壳聚糖硫酸酯衍生物，它可以溶解在许多常见的有机溶剂中，用于对聚醚砜（PES）膜进行修饰。通过元素分析、FTIR、（1）H NMR 和 X 射线衍射图（XRD）来证明功能基团的引入。由于在有机溶剂中的溶解度，壳聚糖的硫酸酯衍生物可以直接与 PES 在有机溶剂中混合，通过液-液相分离技术制备膜。改性膜显示出较低的蛋白质（牛血清白蛋白（BSA）和牛血清纤维蛋白原（BFG））吸附和抑制血小板黏附。此外，与纯 PES 膜相比，改性膜的活化部分凝血活酶时间（APTT）提高了高达 60%。较低的蛋白质吸附、抑制血小板黏附和增加 APTT 证实了壳聚糖硫酸酯衍生物修饰的 PES 膜的血液相容性得到了显著改善。
