2型糖尿病患者与非2型糖尿病患者白内障患者晶状体上皮细胞中PCNA、ICAM-1和波形蛋白的表达。
Expression of PCNA, ICAM-1, and vimentin in lens epithelial cells of cataract patients with and without type 2 diabetes.
作者信息
Fan Haiyan, Suzuki Takahiro, Ogata Masafumi, Nakagawa Yoshihiro, Onouchi Hiromi, Kawai Kenji
机构信息
Department of Ophthalmology, Tokai University School of Medicine, 143 Simokasuya, Isehara, Kanagawa 259-1193, Japan.
出版信息
Tokai J Exp Clin Med. 2012 Jul 20;37(2):51-6.
OBJECTIVE
In order to explore the pathological feature in the progression of cataract with type 2 diabetes, we compared the expression of proliferating cell nuclear antigen (PCNA), intercellular adhesion molecule-1 (ICAM-1), and vimentin in lens epithelial cells (LECs) of cataract patients with and without type 2 diabetes.
METHODS
The indirect immunoperoxidase technique was performed on anterior capsules obtained from 25 patients with type 2 diabetes (DM group) and 25 patients without diabetes (control group). Immunohistochemical difference in the expression of PCNA, ICAM-1, and vimentin in LECs between the 2 groups was investigated.
RESULTS
PCNA expression was decreased (P=0.036) but ICAM-1 expression was significantly increased (P<0.0001) in the DM group as compared with that in the control group. No difference was noted in the expression of vimentin between the 2 groups (P=0.264).
CONCLUSIONS
Decreased proliferation of LECs and increased expression of ICAM-1 in LECs might play an important role in the progression of cataract with type 2 diabetes.
目的
为探讨2型糖尿病患者白内障进展过程中的病理特征，我们比较了伴有和不伴有2型糖尿病的白内障患者晶状体上皮细胞（LECs）中增殖细胞核抗原（PCNA）、细胞间黏附分子-1（ICAM-1）和波形蛋白的表达情况。
方法
对25例2型糖尿病患者（糖尿病组）和25例非糖尿病患者（对照组）的前囊膜进行间接免疫过氧化物酶技术检测。研究两组LECs中PCNA、ICAM-1和波形蛋白表达的免疫组化差异。
结果
与对照组相比，糖尿病组PCNA表达降低（P = 0.036），但ICAM-1表达显著增加（P < 0.0001）。两组波形蛋白表达无差异（P = 0.264）。
结论
LECs增殖减少和LECs中ICAM-1表达增加可能在2型糖尿病患者白内障进展中起重要作用。
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