Darmochwal-Kolarz Dorota, Kolarz Bogdan, Rolinski Jacek, Leszczynska-Gorzelak Bozena, Oleszczuk Jan
Department of Obstetrics and Perinatology, Medical University of Lublin, Poland.
Folia Histochem Cytobiol. 2012 Jul 5;50(2):286-91. doi: 10.5603/fhc.2012.0023.
The aim of our study was to investigate the dynamics of the alterations of soluble human leukocyte antigen-G (sHLA-G) concentrations in sera of healthy non-pregnant women, as well as healthy pregnant women and patients with pre-eclampsia. Thirty five patients with pre-eclampsia, 52 healthy pregnant women, and 24 healthy non-pregnant women were included in the study. Sera concentrations of sHLA-G protein were determined using the immunoenzymatic ELISA method. Statistical analysis was performed using ANOVA and Mann-Whitney U tests. The concentrations of sHLA-G protein in sera of pregnant women in the first, as well as the second and third, trimesters of normal pregnancy were significantly higher in comparison with healthy nonpregnant women. The sera concentrations of sHLA-G in pregnant women in the second trimester of pregnancy were significantly higher compared to the first and third trimesters. The concentrations of sHLA-G in sera of patients with pre-eclampsia were significantly lower than in pregnant women in the third trimester of physiological pregnancy. The results of our study suggest that normal physiological pregnancy is associated with elevated sera concentrations of sHLA-G molecule. The increased concentrations of sHLA-G molecule in mid-gestation could suggest a role for the protein in the second phase of a physiological invasion of extravillous cytotrophoblast to spiral arteries. Furthermore, the results could suggest a role for the decreased sera concentrations of sHLA-G in the pathogenesis of pre-eclampsia.
我们研究的目的是调查健康未孕女性、健康孕妇及先兆子痫患者血清中可溶性人类白细胞抗原-G（sHLA-G）浓度的变化动态。35例先兆子痫患者、52例健康孕妇及24例健康未孕女性纳入本研究。采用免疫酶联ELISA法测定血清中sHLA-G蛋白浓度。使用方差分析和曼-惠特尼U检验进行统计分析。正常妊娠第一、第二和第三孕期孕妇血清中sHLA-G蛋白浓度显著高于健康未孕女性。妊娠中期孕妇血清中sHLA-G浓度显著高于第一和第三孕期。先兆子痫患者血清中sHLA-G浓度显著低于生理性妊娠第三孕期孕妇。我们的研究结果表明，正常生理妊娠与血清中sHLA-G分子浓度升高有关。妊娠中期sHLA-G分子浓度增加可能提示该蛋白在绒毛外细胞滋养层向螺旋动脉生理性侵入的第二阶段发挥作用。此外，结果可能提示血清中sHLA-G浓度降低在先兆子痫发病机制中的作用。
