Rokhafrooz Saber, Ghadiri Ata, Ghandil Pegah, Ghafourian Mehri, Hossaini Seyed Hojjat, Daraei Nahid, Najafian Mahin, Rouhizadeh Ahmad
a Department of Immunology, School of Medicine , Ahvaz Jundishapur University of Medical Sciences , Ahvaz , Iran.
c Department of Medical Genetics, School of Medicine , Ahvaz Jundishapur University of Medical Sciences , Ahvaz , Iran.
Immunol Invest. 2018 Aug;47(6):558-568. doi: 10.1080/08820139.2018.1467925. Epub 2018 Jun 28.
Preeclampsia (PE) is a multisystem syndrome that is a primary source of fetal-maternal morbidity and mortality. Human leukocyte antigen-G (HLA-G) is a nonclassical Major histocompatibility complex (MHC) class-Ib molecule expressed on the extravillous trophoblast and seems to have immunomodulatory functions during pregnancy. The purpose of our study was to investigate whether HLA-G may be a vital marker in the modulation of the pregnancy.
In this case-control study, a number of 150 healthy pregnant women and 150 patients with PE had been genotyped for the 14 base-pair (bp) insertion/deletion polymorphism in exon 8 of the HLA-G gene, and the serum level of soluble HLA-G (sHLA-G) protein was measured using the enzyme-linked immunosorbent assay.
Data showed that the PE syndrome was not related to the HLA-G 14 bp genotype. But, the serum level of sHLA-G in PE patients was significantly lower than that in healthy pregnant women in the third trimester (11.74 and 24.48 U/ml, respectively, p < 0.001). However, no significant association was observed between the HLA-G 14 bp genotype and serum sHLA-G level.
Our results demonstrate that measurement of sHLA-G protein level may be helpful as a primary diagnosis for the pathogenesis of PE. Overall, this study suggests that the association between HLA-G 14 bp polymorphism and serum sHLA-G level in different ethnic populations of PE should be taken into consideration.
子痫前期（PE）是一种多系统综合征，是母婴发病和死亡的主要原因。人类白细胞抗原-G（HLA-G）是一种非经典的主要组织相容性复合体（MHC）I类b分子，表达于绒毛外滋养层，在妊娠期间似乎具有免疫调节功能。我们研究的目的是调查HLA-G是否可能是妊娠调节中的一个重要标志物。
在这项病例对照研究中，对150名健康孕妇和150名单纯性高血压患者进行了HLA-G基因第8外显子14个碱基对（bp）插入/缺失多态性的基因分型，并采用酶联免疫吸附测定法检测可溶性HLA-G（sHLA-G）蛋白的血清水平。
数据显示，子痫前期综合征与HLA-G 14 bp基因型无关。但是，子痫前期患者的血清sHLA-G水平在孕晚期显著低于健康孕妇（分别为11.74和24.48 U/ml，p < 0.001）。然而，未观察到HLA-G 14 bp基因型与血清sHLA-G水平之间存在显著关联。
我们的结果表明，检测sHLA-G蛋白水平可能有助于子痫前期发病机制的初步诊断。总体而言，本研究提示应考虑不同种族子痫前期人群中HLA-G 14 bp多态性与血清sHLA-G水平之间的关联。
