Karbalay-Doust Saied, Noorafshan Ali
Histomorphometry & Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Folia Histochem Cytobiol. 2012 Jul 5;50(2):275-9. doi: 10.5603/fhc.2012.0037.
Changes in the number and size of oocytes can lead to fertilization problems. The present study aimed to evaluate the number, volume, and surface area of oocytes in healthy as well as nandrolone decanoate-treated (ND) mice using stereological methods. Five control mice received vehicle, and five ND-treated mice received ND. Using the 'isotropic Cavalieri' design', the ovary was sectioned. The volume of the ovary (cortex and medulla) was estimated. The oocytes' volume and surface area were estimated using the invariator. The number of the oocytes was estimated using an optical disector. The volumes of the ovary, cortex, and medulla decreased ~50% in the ND-treated mice. The mean number (coefficient of variation) of preantral, antral, and atretic oocytes in the control ovary were 1,690 (0.29), 2,100 (0.52), and 3,900 (0.2), respectively, which decreased ~54%, ~87%, and ~91%, respectively in the ND-treated animals. The mean volume (coefficient of variation) of the preantral, antral, and atretic oocytes were 86,000 (0.27), 110,000 (0.48), and 27,000 (0.33) μm³, respectively. The mean surface area (coefficient of variation) of the three types of oocytes were 9,000 (0.24), 9,900 (0.28), and 4,700 (0.21) μm², respectively. These parameters remained unchanged in the ND-treated mice. ND induces reduction in the number of oocytes, but not in the volume or the surface area.
卵母细胞数量和大小的变化会导致受精问题。本研究旨在采用体视学方法评估健康小鼠以及癸酸诺龙处理（ND）小鼠中卵母细胞的数量、体积和表面积。五只对照小鼠接受赋形剂，五只经ND处理的小鼠接受ND。采用“各向同性卡瓦列里”设计对卵巢进行切片。估计卵巢（皮质和髓质）的体积。使用不变量估计卵母细胞的体积和表面积。使用光学分割器估计卵母细胞的数量。经ND处理的小鼠中，卵巢、皮质和髓质的体积减少了约50%。对照卵巢中窦前、窦状和闭锁卵母细胞的平均数量（变异系数）分别为1690（0.29）、2100（0.52）和3900（0.2），在经ND处理的动物中分别减少了约54%、约87%和约91%。窦前、窦状和闭锁卵母细胞的平均体积（变异系数）分别为86000（0.27）、110000（0.48）和27000（0.33）μm³。三种类型卵母细胞的平均表面积（变异系数）分别为9000（0.24）、9900（0.28）和4700（0.21）μm²。在经ND处理的小鼠中，这些参数保持不变。ND导致卵母细胞数量减少，但不影响其体积或表面积。
