Cherici Camargo Isabel Cristina, Barreiros de Souza Roberta, de Fátima Paccola Mesquita Suzana, Chuffa L G A, Frei F
Department of Biological Sciences, Faculty of Sciences and Letters, São Paulo State University - UNESP, Assis, SP, Brazil.
Acta Biol Hung. 2009 Sep;60(3):253-61. doi: 10.1556/ABiol.60.2009.3.2.
The study was conducted to analyze the histology of the ovaries of adults rats treated with steroids, and submitted or not to physical effort. The control group consisted of females submitted to physical effort and sedentary females, both of which received a physiological solution of 0.9% saline. Treated females, sedentary or not, received 6 mg/kg of body weight of nandrolone decanoate. The steroid and physiological solution were administered intraperitoneally, with a single injection per week for 4 consecutive weeks. The applied physical effort was swimming (20 minutes daily, 5 days/week, for the 4 weeks of treatment). Serial sections (5 mum) of ovaries were prepared for histological evaluation and follicular score. The weight of ovaries and hypophysis, the number of antral and atretic follicles, and the area of corpus luteum were all affected by the steroids. In the ovaries of the control groups, well-developed corpus luteum was observed. In the treated groups, the cortical stroma was occupied by ovarian interstitial tissue. The females treated with steroids presented estral acyclicity. The use of nandrolone decanoate, whether associated with physical effort or not, affected the morphological pattern of the ovaries.
本研究旨在分析用类固醇处理且进行或不进行体力活动的成年大鼠卵巢的组织学情况。对照组由进行体力活动的雌性大鼠和久坐不动的雌性大鼠组成，二者均接受0.9%生理盐水的生理溶液。接受处理的雌性大鼠，无论是否久坐不动，均接受6毫克/千克体重的癸酸诺龙。类固醇和生理溶液通过腹腔注射给药，每周注射一次，连续注射4周。所施加的体力活动为游泳（每天20分钟，每周5天，持续4周的治疗时间）。制备卵巢的连续切片（5微米）用于组织学评估和卵泡评分。卵巢和垂体的重量、窦状卵泡和闭锁卵泡的数量以及黄体面积均受到类固醇的影响。在对照组的卵巢中，观察到发育良好的黄体。在处理组中，皮质基质被卵巢间质组织占据。用类固醇处理的雌性大鼠出现动情周期紊乱。无论是否与体力活动相关，使用癸酸诺龙均会影响卵巢的形态模式。
