Wasiluk Anna, Waszkiewicz Napoleon, Szajda Sławomir Dariusz, Wojewódzka-Żelezniakowicz Marzena, Kępka Alina, Minarowska Alina, Zwierz Zbigniew Wojciech, Pancewicz Sławomir, Ładny Jerzy Robert, Zwierz Krzysztof
Department of Emergency Medicine and Disasters, Medical University of Bialystok, Poland.
Folia Histochem Cytobiol. 2012 Jul 5;50(2):270-4. doi: 10.5603/fhc.2012.0036.
Lyme disease (LD) is the most prevalent tick-borne disease in Europe. LD is caused by the spirochete Borrelia burgdorferi. LD is a chronic disease which can attack a number of organs: skin, heart, brain, joints. Chronic, low-grade inflammation involves general production of pro-inflammatory cytokines and inflammatory markers and is a typical feature of aging. So far, the best method of diagnosing LD is a time-consuming and expensive two-stage serological method. The aim of our study was to evaluate the activity of two lysosomal exoglycosidases: α-fucosidase (FUC) and β-galactosidase (GAL) in the serum of patients with Lyme disease, as potential markers of LD. Due to the increasing number of patients with Lyme disease and a number of false results, new ways to diagnose this disease are still being sought. As elevated level of β-galactosidase is a manifestation of residual lysosomal activity in senescent cells, the increase in its activity in serum during chronic Lyme disease might be a marker of a potentially accelerated senescence process. The study was performed on serum taken from cubital veins of 15 patients with Lyme disease and eight healthy subjects (control group). FUC and GAL activity was measured by the method of Chatterjee et al. as modified by Zwierz et al. In the serum of patients with Lyme disease, GAL activity significantly increased (p = 0.029), and the activity of FUC had a tendency to increase (p = 0.153), compared to the control group. A significant increase in GAL activity in the serum of patients with Lyme disease indicates an increased catabolism of glycoconjugates (glycoproteins, glycolipids, proteoglycans) and could be helpful in the diagnosis of Lyme disease, although this requires confirmation in a larger group of patients. As GAL is the most widely used assay for detection of senescent cells, an elevated level of β-galactosidase might be a manifestation of accelerated senescence process in the course of Lyme disease.
莱姆病（LD）是欧洲最常见的蜱传疾病。莱姆病由螺旋体伯氏疏螺旋体引起。莱姆病是一种慢性疾病，可侵袭多个器官：皮肤、心脏、大脑、关节。慢性低度炎症涉及促炎细胞因子和炎症标志物的普遍产生，是衰老的典型特征。到目前为止，诊断莱姆病的最佳方法是一种耗时且昂贵的两阶段血清学方法。我们研究的目的是评估两种溶酶体外切糖苷酶：α-岩藻糖苷酶（FUC）和β-半乳糖苷酶（GAL）在莱姆病患者血清中的活性，作为莱姆病的潜在标志物。由于莱姆病患者数量不断增加且存在许多假阳性结果，仍在寻找诊断这种疾病的新方法。由于β-半乳糖苷酶水平升高是衰老细胞中溶酶体残余活性的一种表现，慢性莱姆病期间其血清活性增加可能是潜在加速衰老过程的一个标志物。该研究对15例莱姆病患者和8名健康受试者（对照组）肘静脉采集的血清进行。FUC和GAL活性采用Chatterjee等人的方法并经Zwierz等人修改后进行测定。与对照组相比，莱姆病患者血清中GAL活性显著增加（p = 0.029），FUC活性有增加趋势（p = 0.153）。莱姆病患者血清中GAL活性显著增加表明糖缀合物（糖蛋白、糖脂、蛋白聚糖）分解代谢增加，可能有助于莱姆病的诊断，尽管这需要在更大规模的患者群体中得到证实。由于GAL是检测衰老细胞最广泛使用的检测方法，β-半乳糖苷酶水平升高可能是莱姆病病程中加速衰老过程的一种表现。
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