探讨黏着斑调节在人骨髓间充质干细胞成肌分化中的作用。
Insights into the role of focal adhesion modulation in myogenic differentiation of human mesenchymal stem cells.
机构信息
School of Materials Science and Engineering, Nanyang Technological University, Singapore, Singapore.
出版信息
Stem Cells Dev. 2013 Jan 1;22(1):136-47. doi: 10.1089/scd.2012.0160. Epub 2012 Aug 16.
Abstract
We report the establishment of a novel platform to induce myogenic differentiation of human mesenchymal stem cells (hMSCs) via focal adhesion (FA) modulation, giving insights into the role of FA on stem cell differentiation. Micropatterning of collagen type I on a polyacrylamide gel with a stiffness of 10.2 kPa efficiently modulated elongated FA. This elongated FA profile preferentially recruited the β(3) integrin cluster and induced specific myogenic differentiation at both transcription and translation levels with expression of myosin heavy chain and α-sarcomeric actin. This was initiated with elongation of FA complexes that triggered the RhoA downstream signaling toward a myogenic lineage commitment. This study also illustrates how one could partially control myogenic differentiation outcomes of similar-shaped hMSCs by modulating FA morphology and distribution. This technology increases our toolkit choice for controlled differentiation in muscle engineering.
摘要
我们报告了一种通过黏着斑（FA）调节诱导人间质干细胞（hMSCs）成肌分化的新型平台，深入了解了 FA 在干细胞分化中的作用。在 10.2kPa 刚性的聚丙烯酰胺凝胶上对 I 型胶原蛋白进行微图案化，可有效地调节拉长的 FA。这种拉长的 FA 形态优先募集 β(3)整合素簇，并在转录和翻译水平上诱导肌球蛋白重链和 α-横纹肌肌动蛋白的特异性成肌分化。这是通过拉长 FA 复合物触发 RhoA 下游信号开始的，朝着成肌谱系定向。这项研究还说明了如何通过调节 FA 形态和分布来部分控制类似形状的 hMSCs 的成肌分化结果。该技术增加了我们在肌肉工程中进行受控分化的工具选择。
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