IIBBA-CONICET, Química Biológica-FCEyN-Universidad de Buenos Aires and Fundación Instituto Leloir, Buenos Aires, Argentina.
Exp Gerontol. 2012 Jun;47(6):465-72. doi: 10.1016/j.exger.2012.04.001. Epub 2012 Apr 11.
Changes associated with the age-related decline of physiological functions, and their relation with mortality rates, are thoroughly being investigated in the aging research field. We used the Mediterranean fruit fly Ceratitis capitata, largely studied by biodemographers, as a model for functional senescence studies. The aim of our work was to find novel combinatorial indicators able to reflect the functional state of adult insects, regardless of chronological age. We studied the profiles of neutral and polar lipids of head, thorax and abdomen of standard populations kept at 23 °C, at different ages. Lipid classes were separated by thin layer chromatography, and the quantitative values were used to find patterns of change using a multivariate principal component analysis approach. The lipid-dependent principal components obtained correlated with age, and differences between sexes were consistent with differences in the shape of the survival curves and the mortality parameters. These same components were able to discriminate populations with a behavioral decline due to a mild 28 °C thermal stress. Thus, young populations at 28 °C showed similar lipid profiles than old populations at 23 °C. The results indicated that the lipid-dependent components reflect the functional state of the flies, and so were named functional state components (FSCs). It is proposed that FSCs may be used as functional senescence indicators.
在衰老研究领域，人们正在深入研究与生理功能随年龄下降相关的变化及其与死亡率的关系。我们使用广泛的生物人口学家研究的地中海实蝇 Ceratitis capitata 作为功能衰老研究的模型。我们工作的目的是找到新的组合指标，能够反映成年昆虫的功能状态，而不考虑其实际年龄。我们研究了在不同年龄下，保持在 23°C 的标准种群的头部、胸部和腹部的中性和极性脂质的分布。通过薄层色谱法分离脂质类，并使用多元主成分分析方法来寻找变化模式。与年龄相关的脂质依赖性主成分，以及性别差异与生存曲线和死亡率参数的形状差异一致。这些相同的成分能够区分由于轻度 28°C 热应激而导致行为下降的种群。因此，在 28°C 时的年轻种群与在 23°C 时的老年种群具有相似的脂质分布。结果表明，脂质依赖性成分反映了苍蝇的功能状态，因此被命名为功能状态成分（FSCs）。提出 FSCs 可以用作功能衰老的指标。
