Department of Periodontology and Oral Medicine, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
Int J Biol Macromol. 2012 Dec;51(5):738-42. doi: 10.1016/j.ijbiomac.2012.06.035. Epub 2012 Jul 2.
A water-soluble polysaccharide (APP), with a molecular weight of 4.6×10(4) Da, was isolated from Andrographis paniculata and gas chromatography (GC) analysis showed APP was composed of galactose, mannose, fucose, arabinose and rhamnose with molar ratios of 15.4:2.5:4.3:1.5:1.6. The synergetic effect of APP in combination with andrographolide on renal complication in streptozotocin (STZ) induced diabetic mice was investigated. Wistar rats were made diabetic by injection of STZ, and APP and/or andrographolide was administered to diabetic mice for continuous two weeks. APP plus andrographolide not only increased the body weight and creatinine clearance rate (Ccr), but also decreased the levels of serum creatinine, serum urea nitrogen, urinary albumin excretion (UAE), serum urea and blood glucose in diabetic rats, as well as the relative kidney weight. In summary, APP plus andrographolide can improve the metabolic abnormalities of diabetic mice and prevent or delay the progression of diabetic renal complications, which may be useful as a therapeutic agent for inhibiting the progression of diabetic nephropathy.
从穿心莲中分离得到一种水溶性多糖（APP），其分子量为 4.6×10(4)Da。气相色谱（GC）分析表明，APP 由半乳糖、甘露糖、岩藻糖、阿拉伯糖和鼠李糖组成，摩尔比为 15.4:2.5:4.3:1.5:1.6。研究了 APP 与穿心莲内酯联合应用对链脲佐菌素（STZ）诱导的糖尿病小鼠肾脏并发症的协同作用。通过注射 STZ 使 Wistar 大鼠产生糖尿病，并用 APP 和/或穿心莲内酯连续给药 2 周给糖尿病小鼠。APP 加穿心莲内酯不仅增加了体重和肌酐清除率（Ccr），还降低了糖尿病大鼠的血清肌酐、血清尿素氮、尿白蛋白排泄（UAE）、血清尿素和血糖水平，以及相对肾重。总之，APP 加穿心莲内酯可以改善糖尿病小鼠的代谢异常，预防或延缓糖尿病肾病的进展，可能有助于抑制糖尿病肾病的进展。
