Nonnenmann Matthew W, Coronado Gloria, Thompson Beti, Griffith William C, Hanson John Delton, Vesper Stephen, Faustman Elaine M
Department of Occupational and Environmental Health, University of Iowa, Iowa City, Iowa 52242, USA.
J Environ Monit. 2012 Aug;14(8):2038-43. doi: 10.1039/c2em30229b. Epub 2012 Jul 5.
Molecular techniques are an alternative to culturing and counting methods in quantifying indoor fungal contamination. Pyrosequencing offers the possibility of identifying unexpected indoor fungi. In this study, 50 house dust samples were collected from homes in the Yakima Valley, WA. Each sample was analyzed by quantitative PCR (QPCR) for 36 common fungi and by fungal tag-encoded flexible (FLX) amplicon pyrosequencing (fTEFAP) for these and additional fungi. Only 24 of the samples yielded amplified results using fTEFAP but QPCR successfully amplified all 50 samples. Over 450 fungal species were detected by fTEFAP but most were rare. Twenty-two fungi were found by fTEFAP to occur with at least an average of ≥0.5% relative occurrence. Many of these fungi seem to be associated with plants, soil or human skin. Combining fTEFAP and QPCR can enhance studies of fungal contamination in homes.
在量化室内真菌污染方面，分子技术是培养和计数方法的一种替代方法。焦磷酸测序为识别意料之外的室内真菌提供了可能性。在本研究中，从华盛顿州亚基马山谷的家庭中收集了50份房屋灰尘样本。每个样本通过定量PCR（QPCR）分析36种常见真菌，并通过真菌标签编码的弹性（FLX）扩增子焦磷酸测序（fTEFAP）分析这些真菌以及其他真菌。使用fTEFAP时，只有24个样本产生了扩增结果，但QPCR成功扩增了所有50个样本。fTEFAP检测到超过450种真菌，但大多数都很罕见。fTEFAP发现有22种真菌的相对出现率至少平均≥0.5%。这些真菌中的许多似乎与植物、土壤或人类皮肤有关。将fTEFAP和QPCR结合起来可以加强对家庭真菌污染的研究。
