Renz H, Vestner R, Petzoldt S, Brehler C, Prinz H, Rieger C H
Children's Hospital, Marburg a.d. Lahn, FRG.
Int Arch Allergy Appl Immunol. 1990;92(3):247-53. doi: 10.1159/000235185.
Secretory immunoglobulin A (SIgA) anti-casein and SIgA anti-beta-lactoglobulin (BLG) were determined in the saliva of 158 healthy mature infants at birth and in breast milk samples using a direct Elisa technique. IgG anti-casein and anti-BLG were measured in serum samples from mothers and newborns (cord blood). A high risk of allergy was defined in 66 infants who had cord blood (CB)-IgE levels greater than or equal to 0.9 IU/ml and/or parents with atopic diseases. Thirty infants had CB levels less than 0.9 IU/ml and parents without clinical symptoms of atopy but with elevated serum IgE concentrations or type I skin reactions to common allergens (low risk). Sixty-two infants had CB-IgE levels less than 0.9 IU/ml and healthy parents (no risk). The groups were matched for social status, smoking and dietary habits. SIgA anti-casein and anti-BLG were detected in all newborns. SIgA anti-casein was significantly higher (p less than 0.05) in high risk infants (medium 157; 50% confidence limits 45-270) than in no risk (48; 25-150) or low risk infants (43; 21-130). SIgA anti-casein values correlated with maternal allergy, maternal allergy plus CB-IgE, but not with paternal allergy. Breast milk SIgA anti-BLG was depressed (p less than 0.05) in mothers with manifest allergy compared to healthy mothers. Determination of salivary SIgA anti-casein may represent an additional screening method for early detection of infants with atopic disposition.
采用直接酶联免疫吸附测定（ELISA）技术，对158名健康成熟婴儿出生时的唾液以及母乳样本中的分泌型免疫球蛋白A（SIgA）抗酪蛋白和SIgA抗β-乳球蛋白（BLG）进行了测定。同时，对母亲和新生儿（脐带血）的血清样本中的IgG抗酪蛋白和抗BLG进行了检测。66名脐带血（CB）-IgE水平大于或等于0.9 IU/ml和/或父母患有特应性疾病的婴儿被定义为高过敏风险组。30名婴儿的CB水平低于0.9 IU/ml，父母无特应性疾病的临床症状，但血清IgE浓度升高或对常见过敏原呈I型皮肤反应（低风险组）。62名婴儿的CB-IgE水平低于0.9 IU/ml且父母健康（无风险组）。三组在社会地位、吸烟和饮食习惯方面相匹配。所有新生儿均检测到SIgA抗酪蛋白和抗BLG。高风险婴儿的SIgA抗酪蛋白水平（中位数157；50%置信区间45 - 270）显著高于无风险组（48；25 - 150）和低风险组（43；21 - 130）（p < 0.05）。SIgA抗酪蛋白值与母亲过敏、母亲过敏加CB-IgE相关，但与父亲过敏无关。与健康母亲相比，有明显过敏症状的母亲的母乳中SIgA抗BLG水平降低（p < 0.05）。测定唾液SIgA抗酪蛋白可能是早期检测具有特应性体质婴儿的一种额外筛查方法。
