Vassella C C, de Weck A L, Stadler B M
Institute of Clinical Immunology, Inselspital, Bern, Switzerland.
Int Arch Allergy Appl Immunol. 1990;92(3):272-80. doi: 10.1159/000235189.
IgE fragments were detected by polyclonal and monoclonal anti-IgE antibodies using Western blot techniques and immunoassays. Other antibodies detected only intact IgE molecules. In the presence of IgE fragments, higher IgE levels were estimated, depending on the epitope specificity of the antibodies used in the IgE determination assays. IgE fragments have been found together with intact IgE in isolated material from different human sera and also from culture supernatant of an IgE-producing cell line. Our data demonstrate that IgE fragments may be assessed and represent a potential pitfall in diagnostic IgE determinations.
使用蛋白质印迹技术和免疫测定法，通过多克隆和单克隆抗IgE抗体检测IgE片段。其他抗体仅检测完整的IgE分子。在存在IgE片段的情况下，根据IgE测定试验中所用抗体的表位特异性，估计的IgE水平会更高。在来自不同人血清的分离材料以及来自产生IgE的细胞系的培养上清液中，已发现IgE片段与完整的IgE同时存在。我们的数据表明，IgE片段可能会被评估到，并且在诊断性IgE测定中是一个潜在的陷阱。
