天然抗IgE自身抗体会干扰IgE的诊断性检测。
Natural anti-IgE auto-antibodies interfere with diagnostic IgE determination.
作者信息
Vassella C C, de Weck A L, Stadler B M
机构信息
Institute of Clinical Immunology, Inselspital Bern, Switzerland.
出版信息
Clin Exp Allergy. 1990 May;20(3):295-303. doi: 10.1111/j.1365-2222.1990.tb02687.x.
A simple method is described which enables measurement of anti-IgE antibodies in free form as well as in immune complexes of IgE and anti-IgE. Anti-IgE antibodies were purified from serum of one selected blood donor with highly elevated levels of such autoantibodies. These purified anti-IgE auto-antibodies inhibited the measurement of myeloma IgE. Purification also revealed that 98% of the subject's serum IgE was masked by anti-IgE auto-antibodies. Our data suggest that IgE determinations in sera containing anti-IgE antibodies might be underestimated.
本文描述了一种简单的方法，该方法能够测量游离形式以及IgE与抗IgE免疫复合物中的抗IgE抗体。从一名选定的血液供体血清中纯化出抗IgE抗体，该供体血清中此类自身抗体水平极高。这些纯化的抗IgE自身抗体抑制了骨髓瘤IgE的测量。纯化还显示，该受试者血清中98%的IgE被抗IgE自身抗体所掩盖。我们的数据表明，含有抗IgE抗体的血清中IgE的测定结果可能被低估。
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