Banerjee D P, Momin R R, Samantaray S
Department of Veterinary Parasitology, College of veterinary Sciences, Haryana Agricultural University, India.
Int J Parasitol. 1990 Nov;20(7):969-72. doi: 10.1016/0020-7519(90)90037-n.
Studies were conducted on 41 cross-bred (Bos taurus x Bos indicus) calves which were less than 1 year old. Three types of salivary gland antigens (SG Ag) viz. SG Ag-I (whole), SG Ag-II (supernatant) and SG Ag-III (sediment), prepared from the ixodid tick, Hyalomma anatolicum anatolicum, were used for immunizing these calves. The calves were divided into five groups. The first three groups were immunized with SG Ag-I, -II and -III with Freund's complete adjuvant (FCA), respectively. The fourth group was immunized with SG Ag-I (without adjuvant), and the fifth group remained as an unimmunized control inoculated with FCA only. In all the groups, immunization was carried out on days 1 and 14 and the animals were challenged on the 21st day. Significant resistance developed in the calves of the SG Ag-I and SG Ag-II (both with FCA) groups. Of all, SG Ag-I with FCA proved the most potent immunogen. Both humoral and cellular immune responses were demonstrated by in vivo and in vitro tests. The capillary tube agglutination test and double diffusion test gave positive reactions 21 days after first immunization. The immunized calves showed a significant increase in the levels of serum gamma globulin and per cent 'E' rosettes. Tick salivary gland antigens therefore seem to have promising potentials in inducing resistance in calves.
对41头年龄小于1岁的杂交（瘤牛×印度野牛）犊牛进行了研究。从安纳托利亚璃眼蜱制备的三种唾液腺抗原（SG Ag），即SG Ag-I（全抗原）、SG Ag-II（上清液）和SG Ag-III（沉淀物），用于免疫这些犊牛。将犊牛分为五组。前三组分别用弗氏完全佐剂（FCA）与SG Ag-I、-II和-III进行免疫。第四组用SG Ag-I（无佐剂）进行免疫，第五组作为仅接种FCA的未免疫对照。在所有组中，于第1天和第14天进行免疫，并在第21天对动物进行攻毒。SG Ag-I组和SG Ag-II组（均含FCA）的犊牛产生了显著的抵抗力。其中，含FCA的SG Ag-I被证明是最有效的免疫原。通过体内和体外试验证实了体液免疫和细胞免疫反应。首次免疫21天后，毛细管凝集试验和双向扩散试验呈阳性反应。免疫的犊牛血清γ球蛋白水平和“E”玫瑰花环百分比显著增加。因此，蜱唾液腺抗原在诱导犊牛抵抗力方面似乎具有广阔的潜力。
