Banerjee D P, Kumar R, Kumar S, Sengupta P P
Faculty of Veterinary and Animal Sciences, WBUAFS, Kolkata, India.
Trop Anim Health Prod. 2003 Dec;35(6):509-19. doi: 10.1023/a:1027325717124.
Crossbred calves (Bos indicus x Bos taurus) were immunized with a fractionated midgut supernate antigen (GS-F Ag from Hyalomma anatolicum anatolicum). The first inoculation on day 0 was given intramuscularly after emulsification with Freund's complete adjuvant; the second was given subcutaneously on day 14 in incomplete Freund's adjuvant; and the third on day 35 was given subcutaneously without adjuvant. Each injection comprised 1 mg of antigen protein. Ten days after the last inoculation, the immunized calves were challenged simultaneously with 1000 larvae and 20 pairs (20 males and 20 females) of adult H. a. anatolicum on one ear and a similar number of Hyalomma dromedarii ticks on the other ear. There was a significant decrease in the percentage larval engorgement and larval rejection of up to 34% on the immunized calves. A significant increase in the engorgement and preoviposition periods and a significant decrease in the engorged weight, egg mass weight and reproductive index were observed for adult female ticks when fed on the immunized calves. The GS-F Ag also induced a considerable degree of cross-protection in calves against H. dromedarii larval ticks.
用分馏的中肠上清液抗原（来自小亚璃眼蜱的GS-F抗原）对杂交犊牛（瘤牛×黄牛）进行免疫。第0天的首次接种在与弗氏完全佐剂乳化后进行肌肉注射；第14天的第二次接种在不完全弗氏佐剂中进行皮下注射；第35天的第三次接种在无佐剂的情况下进行皮下注射。每次注射包含1毫克抗原蛋白。最后一次接种后10天，对免疫的犊牛进行攻毒，同时在一只耳朵上接种1000只幼虫和20对（20只雄性和20只雌性）成年小亚璃眼蜱，在另一只耳朵上接种数量相似的单峰驼璃眼蜱。免疫的犊牛幼虫饱食率和幼虫排斥率显著降低，最高可达34%。当成年雌性蜱叮咬免疫的犊牛时，观察到饱食期和产卵前期显著延长，饱食体重、卵块重量和繁殖指数显著降低。GS-F抗原还在犊牛中诱导了相当程度的针对单峰驼璃眼蜱幼虫的交叉保护作用。
