Das G, Ghosh S, Khan M H, Sharma J K
Entomology Laboratory, Division of Parasitology, Indian Veterinary Research Institute, Uttar Pradesh.
Exp Appl Acarol. 2000 Aug;24(8):645-59. doi: 10.1023/a:1026581200514.
Extracts prepared from unfed larvae of Hyalomma anatolicum anatolicum were purified by immunoaffinity chromatography using anti-gut IgG as ligand. Affinity purified antigen (Aff-GHLAg) was used to immunize cross-bred (Bos taurus x B. indicus) calves of 6-7 months of age. Immunized calves rejected 70.6% larvae, 54.5% nymphs and 61.9% adults. No significant changes in the engorged weight of females was observed; however, significant decrease in the engorgement weight of larvae and nymphs was recorded. There was a significant decrease in the emerging nymphs (p < 0.05) and adults (p < 0.01) of the tick stages fed on immunized animals. SDS-PAGE analysis revealed three antigenic proteins of 100, 59.4 and 37 kDa responsible for induction of resistance in the host.
用抗肠道IgG作为配体，通过免疫亲和层析法对土耳其小亚璃眼蜱未进食幼虫制备的提取物进行纯化。用亲和纯化抗原（Aff-GHLAg）免疫6至7月龄的杂交（黄牛×印度瘤牛）犊牛。免疫后的犊牛拒斥了70.6%的幼虫、54.5%的若虫和61.9%的成虫。未观察到雌性蜱饱血重量有显著变化；然而，记录到幼虫和若虫的饱血重量显著下降。取食免疫动物的蜱各阶段羽化出的若虫（p<0.05）和成虫（p<0.01）数量显著减少。SDS-PAGE分析显示，有三种抗原蛋白，分子量分别为100、59.4和37 kDa，它们可诱导宿主产生抗性。
