肌动蛋白信使核糖核酸（Actin mRNA）在没有蛋白质合成的情况下进行定位。
Actin mRNA localizes in the absence of protein synthesis.
作者信息
Sundell C L, Singer R H
机构信息
Department of Cell Biology, University of Massachusetts Medical School, Worcester 01655.
出版信息
J Cell Biol. 1990 Dec;111(6 Pt 1):2397-403. doi: 10.1083/jcb.111.6.2397.
Actin mRNA is localized in chicken embryo fibroblasts to the distal regions of leading lamellae, but not within the ruffling edges. In this investigation we have addressed the role of actin translation in this process. The translocation of actin mRNA to the cell periphery was studied by monitoring the distribution of actin mRNA in cells during spreading. Within 90 min, actin mRNA moved from a perinuclear to a peripheral distribution. Formation of lamellipodia preceded actin mRNA localization, indicating that localization is not a prerequisite for this event. Neither puromycin (which dissociates ribosomes from mRNA) nor cycloheximide (which stabilizes ribosomes on mRNA) had any effect on this movement of actin mRNA. Anchoring of actin mRNA was studied using cells with peripherally localized actin mRNA. No change in actin mRNA localization was observed for 30 min in the same inhibitors. These data indicate that the presence of the nascent polypeptide is not necessary for translocation of actin mRNA to the cell periphery, or anchoring at that site. This suggests that the mRNA contains information concerning its spatial distribution within the cytoplasm.
肌动蛋白信使核糖核酸（Actin mRNA）在鸡胚成纤维细胞中定位于前缘薄板的远端区域，但不在边缘波动处。在本研究中，我们探讨了肌动蛋白翻译在此过程中的作用。通过监测铺展过程中细胞内肌动蛋白信使核糖核酸的分布，研究了其向细胞周边的转运。在90分钟内，肌动蛋白信使核糖核酸从核周分布转移到周边分布。片状伪足的形成先于肌动蛋白信使核糖核酸的定位，这表明定位不是该事件的先决条件。嘌呤霉素（使核糖体与信使核糖核酸解离）和环己酰亚胺（使核糖体稳定在信使核糖核酸上）对肌动蛋白信使核糖核酸的这种移动均无影响。使用肌动蛋白信使核糖核酸定位于周边的细胞研究了其锚定情况。在相同抑制剂作用下30分钟内，未观察到肌动蛋白信使核糖核酸定位的变化。这些数据表明，新生多肽的存在对于肌动蛋白信使核糖核酸向细胞周边的转运或在该位点的锚定并非必需。这表明信使核糖核酸包含有关其在细胞质内空间分布的信息。
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