Department of Biochemistry, University of Oxford, Oxford, UK.
Weatherall Institute for Molecular Medicine, University of Oxford , Oxford, UK.
J Cell Biol. 2023 Jun 5;222(6). doi: 10.1083/jcb.202205129. Epub 2023 May 5.
While post-transcriptional control is thought to be required at the periphery of neurons and glia, its extent is unclear. Here, we investigate systematically the spatial distribution and expression of mRNA at single molecule sensitivity and their corresponding proteins of 200 YFP trap lines across the intact Drosophila nervous system. 97.5% of the genes studied showed discordance between the distribution of mRNA and the proteins they encode in at least one region of the nervous system. These data suggest that post-transcriptional regulation is very common, helping to explain the complexity of the nervous system. We also discovered that 68.5% of these genes have transcripts present at the periphery of neurons, with 9.5% at the glial periphery. Peripheral transcripts include many potential new regulators of neurons, glia, and their interactions. Our approach is applicable to most genes and tissues and includes powerful novel data annotation and visualization tools for post-transcriptional regulation.
虽然人们认为转录后调控发生在神经元和神经胶质细胞的外围，但具体程度尚不清楚。在这里，我们系统地研究了 200 个 YFP 捕获系在完整果蝇神经系统中单分子灵敏度下的 mRNA 及其相应蛋白的空间分布和表达。在所研究的基因中，有 97.5%的基因在神经系统的至少一个区域显示出 mRNA 与它们编码的蛋白质之间的分布不一致。这些数据表明，转录后调控非常普遍，有助于解释神经系统的复杂性。我们还发现，这些基因中有 68.5%的转录本存在于神经元的外围，9.5%的转录本存在于神经胶质细胞的外围。外围转录本包括许多潜在的神经元、神经胶质及其相互作用的新调控因子。我们的方法适用于大多数基因和组织，并包含用于转录后调控的强大的新型数据注释和可视化工具。
