对清醒大鼠松果体微透析液中的褪黑素进行同步在线采样，并通过高效液相色谱-电化学检测法进行分析。
Concurrent on-line sampling of melatonin in pineal microdialysates from conscious rat and its analysis by high-performance liquid chromatography with electrochemical detection.
作者信息
Azekawa T, Sano A, Aoi K, Sei H, Morita Y
机构信息
Department of Physiology, School of Medicine, University of Tokushima, Japan.
出版信息
J Chromatogr. 1990 Aug 24;530(1):47-55. doi: 10.1016/s0378-4347(00)82301-4.
Dynamic changes of melatonin in microdialysates from the pineal gland of a freely moving rat were repeatedly determined by using on-line high-performance liquid chromatography with electrochemical detection. The detection limit for melatonin, ca. 5 pg, was well below that achieved with other systems. We observed a drastic increase of extracellular pineal melatonin during the transitional phase from the light period to the dark period. This application of microdialysis is a useful tool in the study of the physiological role of the mammalian pineal body.
采用在线高效液相色谱-电化学检测法，反复测定了自由活动大鼠松果体微透析液中褪黑素的动态变化。褪黑素的检测限约为5皮克，远低于其他系统所能达到的检测限。我们观察到，在从光照期向黑暗期过渡的阶段，细胞外松果体褪黑素急剧增加。这种微透析应用是研究哺乳动物松果体生理作用的一种有用工具。
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