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从埃塞俄比亚人体头虱和体虱中检测到鲍曼不动杆菌,并鉴定出新的基因型。

Detection of Acinetobacter baumannii in human head and body lice from Ethiopia and identification of new genotypes.

机构信息

URMITE, UMR IRD 198/CNRS 6236, Faculté de Médecine, 27 Bd Jean Moulin, 13385 Marseille cedex 05, France.

出版信息

Int J Infect Dis. 2012 Sep;16(9):e680-3. doi: 10.1016/j.ijid.2012.05.1024. Epub 2012 Jul 6.

Abstract

BACKGROUND

Acinetobacter baumannii has previously been detected and genotyped in human body lice. The objectives of this study were to determine the presence of this bacterium in head and body lice collected from healthy individuals in Ethiopia by molecular methods and to characterize the genotype.

METHODS

Human lice from locations at different altitudes in Ethiopia were screened for the presence of Acinetobacter sp by targeting the rpoB gene. Acinetobacter baumannii was detected and genotyped using recA PCR amplification.

RESULTS

A total of 115 head and 109 body lice were collected from 134 healthy individuals. Acinetobacter sp were found in 54 head (47%) and 77 body (71%) lice. The recA gene was sequenced for 60 of the Acinetobacter sp and 67% were positive for A. baumannii; genotype 1 was retrieved the most frequently.

CONCLUSION

Our study is the first to show the presence of A. baumannii in human body lice, and also in head lice, in Ethiopia.

摘要

背景

鲍曼不动杆菌先前已在人体虱中被检测和基因分型。本研究的目的是通过分子方法确定从埃塞俄比亚健康个体中采集的头虱和体虱中是否存在这种细菌,并对其基因型进行特征描述。

方法

通过针对 rpoB 基因,对来自埃塞俄比亚不同海拔地区的人体虱进行针对不动杆菌属的筛查。使用 recA PCR 扩增检测和基因分型鲍曼不动杆菌。

结果

从 134 名健康个体中采集了 115 个头虱和 109 个体虱。在 54 个头虱(47%)和 77 个体虱(71%)中发现了不动杆菌属。对 60 株不动杆菌属的 recA 基因进行了测序,其中 67%为鲍曼不动杆菌阳性;最常检出的基因型为 1 型。

结论

本研究首次表明在埃塞俄比亚的人体虱中存在鲍曼不动杆菌,也存在于头虱中。

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