为了提高生物技术中的生产性能,对真菌形态进行特征描述和控制。

Characterization and control of fungal morphology for improved production performance in biotechnology.

机构信息

Institute of Biochemical Engineering, Technische Universität Braunschweig, Germany.

出版信息

J Biotechnol. 2013 Jan 20;163(2):112-23. doi: 10.1016/j.jbiotec.2012.06.024. Epub 2012 Jul 4.

Abstract

Filamentous fungi have been widely applied in industrial biotechnology for many decades. In submerged culture processes, they typically exhibit a complex morphological life cycle that is related to production performance--a link that is of high interest for process optimization. The fungal forms can vary from dense spherical pellets to viscous mycelia. The resulting morphology has been shown to be influenced strongly by process parameters, including power input through stirring and aeration, mass transfer characteristics, pH value, osmolality and the presence of solid micro-particles. The surface properties of fungal spores and hyphae also play a role. Due to their high industrial relevance, the past years have seen a substantial development of tools and techniques to characterize the growth of fungi and obtain quantitative estimates on their morphological properties. Based on the novel insights available from such studies, more recent studies have been aimed at the precise control of morphology, i.e., morphology engineering, to produce superior bio-processes with filamentous fungi.

摘要

丝状真菌在工业生物技术领域已经得到了广泛应用数十年。在液体深层发酵过程中,它们通常表现出与生产性能相关的复杂形态生命周期——这一联系是工艺优化的重点。真菌的形态可以从密集的球形颗粒到粘性的菌丝体变化。研究表明,这些形态强烈地受到工艺参数的影响,包括搅拌和通气带来的功率输入、传质特性、pH 值、渗透压以及固体微粒的存在。真菌孢子和菌丝的表面特性也发挥了作用。由于其具有高度的工业相关性,近年来已经开发出大量的工具和技术来对真菌的生长进行表征,并对其形态特性进行定量估计。基于这些研究提供的新见解,最近的研究旨在对形态进行精确控制,即形态工程,以利用丝状真菌生产更优的生物工艺。

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