College of Marine Life Sciences, Ocean University of China, Qingdao, PR China.
Int J Syst Evol Microbiol. 2013 Apr;63(Pt 4):1235-1240. doi: 10.1099/ijs.0.041509-0. Epub 2012 Jul 6.
A novel Gram-stain-negative, rod-shaped, non-flagellated, strictly aerobic strain with gliding motility, designated SW024(T), was isolated from surface seawater of the South Pacific Gyre (26° 29' S 137° 56' W) during the Integrated Ocean Drilling Program Expedition 329. Phylogenetic analyses based on 16S rRNA gene sequences showed that the novel strain was related most closely to Aquimarina muelleri KMM 6020(T), Aquimarina macrocephali JAMB N27(T), Aquimarina addita JC2680(T), Aquimarina mytili PSC33(T), Aquimarina intermedia KMM 6258(T), Aquimarina latercula ATCC 23177(T), Aquimarina spongiae A6(T), Aquimarina agarilytica ZC1(T) and Aquimarina brevivitae SMK-19(T) (96.1, 95.5, 95.0, 94.4, 94.3, 94.0, 93.4, 93.3 and 93.2 % similarities, respectively), demonstrating that the novel strain belonged to the genus Aquimarina. The DNA G+C content of strain SW024(T) was 30.8 mol%. The major respiratory quinone of strain SW024(T) was MK-6. The dominant fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 1 G, C16 : 0 10-methyl and/or iso-C17 : 1ω9c, and iso-C15 : 0 3-OH. The polar lipids of strain SW024(T) were phosphatidylethanolamine, three unknown aminolipids and five unknown polar lipids. On the basis of combined phenotypic and phylogenetic analyses, strain SW024(T) represents a novel species of the genus Aquimarina, for which the name Aquimarina longa sp. nov. is proposed. The type strain is SW024(T) ( = CGMCC 1.11007(T) = JCM 17859(T)). An emended description of A. muelleri is also proposed.
一株新型革兰氏阴性、杆状、无鞭毛、严格需氧、具有滑行运动能力的菌株，SW024(T)，从南太平洋 Gyre（26° 29' S 137° 56' W）的表层海水中分离出来，这是在综合大洋钻探计划第 329 航次期间进行的。基于 16S rRNA 基因序列的系统发育分析表明，该新菌株与 Aquimarina muelleri KMM 6020(T)、Aquimarina macrocephali JAMB N27(T)、Aquimarina addita JC2680(T)、Aquimarina mytili PSC33(T)、Aquimarina intermedia KMM 6258(T)、Aquimarina latercula ATCC 23177(T)、Aquimarina spongiae A6(T)、Aquimarina agarilytica ZC1(T) 和 Aquimarina brevivitae SMK-19(T)（分别为 96.1%、95.5%、95.0%、94.4%、94.3%、94.0%、93.4%、93.3%和 93.2%的相似性）最为密切相关，表明该新菌株属于 Aquimarina 属。SW024(T)株的 DNA G+C 含量为 30.8 mol%。SW024(T)株的主要呼吸醌为 MK-6。优势脂肪酸为 iso-C15:0、iso-C17:0 3-OH、iso-C15:1 G、C16:0 10-methyl 和/或 iso-C17:1ω9c 和 iso-C15:0 3-OH。SW024(T)株的极性脂为磷脂酰乙醇胺、三种未知的氨基脂和五种未知的极性脂。基于表型和系统发育综合分析，SW024(T)株代表 Aquimarina 属的一个新种，建议命名为 Aquimarina longa sp. nov.。模式株为 SW024(T)（ = CGMCC 1.11007(T) = JCM 17859(T)）。还提出了对 Aquimarina muelleri 的修订描述。
