Department of Biology, College of Natural Science, Jeju National University, Jeju 690-756, Republic of Korea.
The Research Institute for Basic Sciences, Jeju National University, Jeju 690-756, Republic of Korea.
Int J Syst Evol Microbiol. 2011 Feb;61(Pt 2):417-421. doi: 10.1099/ijs.0.022046-0. Epub 2010 Mar 26.
A Gram-stain-negative, yellow-pigmented, rod-shaped, strictly aerobic, non-flagellated, non-gliding and oxidase- and catalase-positive bacterium, designated A6(T), was isolated from a marine sponge, Halichondria oshoro, collected on the coast of Jeju Island, South Korea. Phylogenetic analysis based on the nearly complete 16S rRNA gene sequence revealed that strain A6(T) was a member of the family Flavobacteriaceae. The closest relatives were Aquimarina intermedia LMG 23204(T), A. latercula ATCC 23177(T), A. brevivitae SMK-19(T) and A. muelleri KMM 6020(T), with which strain A6(T) shared 95.7, 95.1, 94.7 and 94.6 % 16S rRNA gene sequence similarity, respectively. The dominant fatty acids of strain A6(T) were iso-C(15 : 0) (32.2 %), iso-C(17 : 0) 3-OH (20.0 %), iso-C(15 : 0) 3-OH (12.3 %), iso-C(15 : 1) G (7.2 %) and summed feature 3 (comprising iso-C(15 : 0) 2-OH and/or C(16 : 1)ω7c; 6.8 %). The DNA G+C content of strain A6(T) was 36.0 mol% and the major respiratory quinone was MK-6. On the basis of combined phenotypic and phylogenetic analyses, strain A6(T) represents a novel species of the genus Aquimarina, for which the name Aquimarina spongiae sp. nov. is proposed. The type strain is A6(T) (=KCTC 22663(T) =DSM 22623(T)).
一株革兰氏阴性、黄色着色、杆状、严格需氧、无鞭毛、不滑行、氧化酶和过氧化氢酶阳性的细菌，被命名为 A6(T)，从韩国济州岛海岸采集的海洋海绵 Halichondria oshoro 中分离得到。基于近完整 16S rRNA 基因序列的系统发育分析表明，菌株 A6(T)是黄杆菌科的一个成员。其最接近的亲缘关系是 Aquimarina intermedia LMG 23204(T)、A. latercula ATCC 23177(T)、A. brevivitae SMK-19(T)和 A. muelleri KMM 6020(T)，与菌株 A6(T)的 16S rRNA 基因序列相似性分别为 95.7%、95.1%、94.7%和 94.6%。菌株 A6(T)的主要脂肪酸为 iso-C(15:0) (32.2%)、iso-C(17:0) 3-OH (20.0%)、iso-C(15:0) 3-OH (12.3%)、iso-C(15:1) G (7.2%)和 sum feature 3 (由 iso-C(15:0) 2-OH 和/或 C(16:1)ω7c 组成；6.8%)。菌株 A6(T)的 DNA G+C 含量为 36.0 mol%，主要呼吸醌为 MK-6。基于综合表型和系统发育分析，菌株 A6(T)代表了 Aquimarina 属的一个新种，建议将其命名为 Aquimarina spongiae sp. nov.。模式菌株为 A6(T) (=KCTC 22663(T)=DSM 22623(T))。
