Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 540-742, Republic of Korea.
Int J Syst Evol Microbiol. 2013 May;63(Pt 5):1782-1787. doi: 10.1099/ijs.0.044289-0. Epub 2012 Aug 31.
An orange-coloured and slender rod-shaped bacterium, designated strain PSC32(T), was isolated from the gut microflora of a mussel collected from Gwangyang Bay, South Sea (Republic of Korea). Cells were Gram-reaction-negative, strictly aerobic, and catalase- and oxidase-positive. The major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 1 G and iso-C15 : 0 3-OH. The only isoprenoid quinone of strain PSC32(T) was MK-6 and the DNA G+C content was 36.9 mol%. Phosphatidylethanolamine, one unidentified aminolipid and two unidentified polar lipids were found as major polar lipids. A phylogenetic tree based on 16S rRNA gene sequences showed that strain PSC32(T) forms an evolutionary lineage within the genus Aquimarina and is closely related to Aquimarina spongiae A6(T) (97.0 % similarity) and to other members of the genus Aquimarina (94.4-96.5 %). Genomic DNA-DNA relatedness between strain PSC32(T) and A. spongiae A6(T) was 40.7 %. A number of phenotypic characteristics distinguished strain PSC32(T) from described members of the genus Aquimarina. On the basis of the evidence presented in this study, strain PSC32(T) represents a novel species, for which the name Aquimarina gracilis sp. nov. is proposed. The type strain is PSC32(T) ( = KCTC 23301(T) = JCM 17453(T)). An emended description of Aquimarina spongiae is given.
一株橙色、细长的杆状细菌，被命名为PSC32(T) 菌株，从韩国南海光阳湾采集的贻贝肠道微生物中分离得到。细胞革兰氏反应阴性，严格需氧，过氧化氢酶和氧化酶阳性。主要脂肪酸为 iso-C15：0、iso-C17：0 3-OH、iso-C15：1 G 和 iso-C15：0 3-OH。PSC32(T) 株的唯一异戊烯醌为 MK-6，DNA G+C 含量为 36.9 mol%。鉴定出的主要极性脂类有磷脂酰乙醇胺、一种未鉴定的胺脂和两种未鉴定的极性脂类。基于 16S rRNA 基因序列构建的系统发育树显示，PSC32(T) 菌株在 Aquimarina 属内形成一个进化分支，与 Aquimarina spongiae A6(T)（相似度 97.0%）和 Aquimarina 属的其他成员（相似度 94.4-96.5%）关系最为密切。PSC32(T) 菌株与 A. spongiae A6(T) 之间的基因组 DNA-DNA 同源性为 40.7%。PSC32(T) 菌株与已描述的 Aquimarina 属成员有许多表型特征不同。根据本研究提供的证据，PSC32(T) 菌株代表一个新的物种，命名为 Aquimarina gracilis sp. nov. 。模式菌株为 PSC32(T)（=KCTC 23301(T)=JCM 17453(T)）。对 Aquimarina spongiae 的描述进行了修订。
