College of Marine Life Sciences, Ocean University of China, Qingdao, China.
Int J Syst Evol Microbiol. 2014 Jan;64(Pt 1):122-127. doi: 10.1099/ijs.0.055517-0. Epub 2013 Sep 12.
A novel Gram-stain-negative, rod-shaped, non-flagellated, strictly aerobic strain with gliding motility, designated XH134(T), was isolated from surface seawater of the South Pacific Gyre (45° 58' S 163° 11' W) during the Integrated Ocean Drilling Program Expedition 329. The major respiratory quinone of strain XH134(T) was MK-6. The dominant fatty acids of strain XH134(T) were iso-C15 : 0, iso-C15 : 1 G, C16 : 1ω6c and/or C16 : 1ω7c, iso-C17 : 0 3-OH, iso-C15 : 0 3-OH and 10-methyl C16 : 0 and/or iso-C17 : 1ω9c. The polar lipids of strain XH134(T) comprised phosphatidylethanolamine, one unknown aminolipid and three unknown polar lipids. The DNA G+C content of strain XH134(T) was 32.4 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that the novel strain was related most closely to Aquimarina macrocephali JAMB N27(T) with 96.9 % sequence similarity. A number of phenotypic characteristics distinguished strain XH134(T) from described members of the genus Aquimarina. On the basis of combined phenotypic and phylogenetic analyses, strain XH134(T) represents a novel species of the genus Aquimarina, for which the name Aquimarina megaterium sp. nov. is proposed. The type strain is XH134(T) ( = CGMCC 1.12186(T) = JCM 18215(T)).
一株新型革兰氏阴性、杆状、不具鞭毛、严格需氧、具有滑行运动能力的海洋细菌 XH134(T)，是从南太平洋 Gyre（45°58'S 163°11'W）的表层海水中，通过综合大洋钻探计划 329 航次分离得到的。XH134(T)菌株的主要呼吸醌是 MK-6。XH134(T)菌株的优势脂肪酸为 iso-C15:0、iso-C15:1 G、C16:1ω6c 和/或 C16:1ω7c、iso-C17:0 3-OH、iso-C15:0 3-OH 和 10-甲基 C16:0 和/或 iso-C17:1ω9c。XH134(T)菌株的极性脂包括磷脂酰乙醇胺、一种未知的氨基脂和三种未知的极性脂。XH134(T)菌株的 DNA G+C 含量为 32.4 mol%。基于 16S rRNA 基因序列的系统发育分析表明，该新菌株与 Aquimarina macrocephali JAMB N27(T)的亲缘关系最近，相似度为 96.9%。一些表型特征将菌株 XH134(T)与描述的 Aquimarina 属成员区分开来。基于综合表型和系统发育分析，菌株 XH134(T)代表 Aquimarina 属的一个新种，现命名为 Aquimarina megaterium sp. nov.。其模式菌株为 XH134(T)（=CGMCC 1.12186(T)=JCM 18215(T)）。
