Analytical Chemistry Division, Hollings Marine Laboratory, National Institute of Standards and Technology, Charleston, SC 29412, USA.
Anal Bioanal Chem. 2012 Aug;404(3):777-84. doi: 10.1007/s00216-012-6169-2. Epub 2012 Jul 8.
Nuclear magnetic resonance (NMR) spectroscopy has been used to obtain metabolic profiles of the polar diatom Fragilariopsis cylindrus, leading to the identification of a novel metabolite in this organism. Initial results from an ongoing metabolomics study have led to the discovery of isethionic acid (2-hydroxyethanesulfonic acid, CAS: 107-36-8) as a major metabolite in F. cylindrus. This compound is being produced by the organism under normal culture conditions. This finding is the first report of a diatom producing isethionic acid. In addition to isethionic acid, four other metabolites, dimethylsulfoniopropionate (DMSP), betaine, homarine, and proline were present and may serve as osmoprotectants in F. cylindrus. NMR-based metabolite profiles of F. cylindrus were obtained along a growth curve of the organism. The relative concentration levels of the five metabolites were monitored over a growth period of F. cylindrus from 18 to 25 days. All showed an increase in relative concentration with time, except for proline, which began to decrease after day 21.
核磁共振（NMR）光谱已被用于获取极地硅藻脆杆藻的代谢物图谱，从而在该生物体内鉴定出一种新的代谢物。正在进行的代谢组学研究的初步结果导致发现异硫氨酸（2-羟乙磺酸，CAS：107-36-8）是脆杆藻的主要代谢物。该化合物是在正常培养条件下由该生物产生的。这一发现是关于硅藻产生异硫氨酸的首次报道。除了异硫氨酸，还存在另外四种代谢物，即二甲基亚砜丙酸盐（DMSP）、甜菜碱、高丙氨酸和脯氨酸，它们可能在脆杆藻中作为渗透保护剂。我们获得了沿着该生物生长曲线的脆杆藻的基于 NMR 的代谢物图谱。在从 18 天到 25 天的脆杆藻生长期间，监测了这五种代谢物的相对浓度水平。除了脯氨酸外，所有代谢物的相对浓度都随时间增加，脯氨酸在第 21 天后开始减少。
