Cellzome AG, Meyerhofstrasse 1, 69117, Heidelberg, Germany.
Anal Bioanal Chem. 2012 Sep;404(4):939-65. doi: 10.1007/s00216-012-6203-4. Epub 2012 Jul 8.
Mass-spectrometry-based proteomics is continuing to make major contributions to the discovery of fundamental biological processes and, more recently, has also developed into an assay platform capable of measuring hundreds to thousands of proteins in any biological system. The field has progressed at an amazing rate over the past five years in terms of technology as well as the breadth and depth of applications in all areas of the life sciences. Some of the technical approaches that were at an experimental stage back then are considered the gold standard today, and the community is learning to come to grips with the volume and complexity of the data generated. The revolution in DNA/RNA sequencing technology extends the reach of proteomic research to practically any species, and the notion that mass spectrometry has the potential to eventually retire the western blot is no longer in the realm of science fiction. In this review, we focus on the major technical and conceptual developments since 2007 and illustrate these by important recent applications.
基于质谱的蛋白质组学继续为基础生物学过程的发现做出重大贡献，最近也发展成为一种能够在任何生物系统中测量数百到数千种蛋白质的分析平台。在过去五年中，该领域在技术以及生命科学各个领域的应用广度和深度方面都取得了惊人的进展。当时处于实验阶段的一些技术方法现在被认为是金标准，并且该领域正在学习如何应对生成的数据的数量和复杂性。DNA/RNA 测序技术的革命将蛋白质组学研究的范围扩展到几乎所有物种，并且质谱法有可能最终取代蛋白质印迹法的观点已不再是科幻小说。在这篇综述中，我们重点介绍了 2007 年以来的主要技术和概念发展，并通过最近的一些重要应用来说明这些发展。
