Biemann Klaus
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
J Proteomics. 2014 Jul 31;107:62-70. doi: 10.1016/j.jprot.2014.01.008. Epub 2014 Jan 18.
The development of mass spectrometric methods in peptide and protein chemistry in the author's laboratory is reviewed, from the first determination of the amino acid sequence of small peptides in the late 1950s to its use for the determination of the primary structure of large proteins by a combination of mass spectrometry and DNA sequencing in the late 1980s. This article is part of a Special Issue entitled: 20years of Proteomics in memory of Viatliano Pallini. Guest Editors: Luca Bini, Juan J. Calvete, Natacha Turck, Denis Hochstrasser and Jean-Charles Sanchez.
本文回顾了作者实验室在肽和蛋白质化学领域质谱方法的发展历程，从20世纪50年代末首次测定小肽的氨基酸序列，到80年代末通过质谱和DNA测序相结合的方法用于测定大蛋白质的一级结构。本文是题为“纪念维亚利阿诺·帕利尼的蛋白质组学20年”特刊的一部分。客座编辑：卢卡·比尼、胡安·J·卡尔维特、娜塔莎·图尔克、丹尼斯·霍赫施塔瑟和让-查尔斯·桑切斯。
