多囊卵巢综合征患者卵丘细胞中核糖体 RNA 的过表达。
Overexpression of ribosomal RNA in cumulus cells of patients with polycystic ovary syndrome.
机构信息
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya St, 117997 Moscow, Russia.
出版信息
J Assist Reprod Genet. 2012 Oct;29(10):1141-5. doi: 10.1007/s10815-012-9827-6. Epub 2012 Jul 7.
OBJECTIVE
To evaluate the level of ribosomal RNA (rRNA) in cumulus cells (CCs) from women with polycystic ovary syndrome (PCOS) undergoing ICSI.
MATERIALS AND METHODS
The study population included three healthy oocyte donors (control) and three patients with PCOS. RNA expression in CCs was assessed using quantitative real-time PCR assay to measure the pre-rRNA transcripts (5'ETS region), 18 S, 5.8 S and 28 S rRNA.
RESULTS
The level of 18 S rRNA is 3.9 times higher (p = 0.036) and the level of 5.8 S rRNA is 2.9 times higher (p = 0.049) in CCs of PCOS patients than in CCs of healthy women. The fold change in expression of 28 S rRNA in CCs of PCOS patients also exceeds that in the control group, but did not reach a statistical significance (p = 0.342).
CONCLUSION
Our observations support the idea that CCs of PCOS patients contain comparatively more ribosomes that CCs of healthy oocyte donors that may indicate a higher proliferation rate or up-regulated translation of protein factors in ССs of PCOS patients.
目的
评估接受卵胞浆内单精子注射（ICSI）的多囊卵巢综合征（PCOS）患者的卵丘细胞（CC）中核糖体 RNA（rRNA）的水平。
材料与方法
研究人群包括三名健康的卵母细胞供体（对照组）和三名 PCOS 患者。使用实时定量 PCR 检测评估 CC 中的 RNA 表达，以测量前 rRNA 转录本（5'ETS 区）、18S、5.8S 和 28S rRNA。
结果
PCOS 患者的 CC 中 18S rRNA 的水平高 3.9 倍（p=0.036），5.8S rRNA 的水平高 2.9 倍（p=0.049）。PCOS 患者的 CC 中 28S rRNA 的表达倍数变化也高于对照组，但未达到统计学意义（p=0.342）。
结论
我们的观察结果支持这样一种观点，即 PCOS 患者的 CC 中含有相对较多的核糖体，这可能表明 PCOS 患者的 CC 中存在更高的增殖率或蛋白质因子的翻译上调。
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