Rohn Matthew C H, Simeone Jocelyn M, Doctorman Steven, Ge Peixi, Hernandez Ana, Das Samarjit, Spann Marcus, Dow Mark, Segars James, Singh Bhuchitra
Department of Obstetrics and Gynecology, George Washington University Hospital, Washington, DC, USA.
University of Georgia, Athens, GA, USA.
Reprod Sci. 2025 Aug 6. doi: 10.1007/s43032-025-01940-5.
A systematic review was conducted pertaining to the biologic processes of cumulus cells and measures of oocyte quality. The initial search yielded 18,549 articles, and after screening, 65 studies were included in this review. Extracted data were synthesized descriptively. Articles were categorized based on the biologic feature of cumulus cells addressed: genetic expression, biochemical activity, and morphologic characteristics. Manuscripts addressing cumulus cell function in association with gynecologic conditions and their impact on oocyte quality were also reviewed. Findings from six studies showed an association between increased expression of pathways related to extracellular matrix development (HAS2, VCAN) and improved oocyte quality. Six manuscripts reported improved oocyte quality related to genes involved in cumulus cell expansion (GREM1, PTGS2) and 6 articles noted patterns in expression of regulators of apoptotic pathways (BCL2, BIRC5, MDM2, PGAM5). Twelve studies examined biochemical features of cumulus cells, including cumulus mitochondrial membrane resistance, respirometric index, and acetyl-CoA levels, which have been associated with oocyte development. Morphologic assessment of cumulus cells was evaluated by 5 studies, demonstrating a relationship between mitochondrial dimensions, telomere length, and cumulus density/dispersion with oocyte quality. Twenty-two articles assessed cumulus cell function and its relationship with oocyte quality in diseases such as polycystic ovarian syndrome (PCOS) and endometriosis conditions, and increased maternal age (with or without decreased ovarian reserve). This comprehensive systematic review has integrated findings from studies investigating the relationship between cumulus cells and oocyte quality, identifying patterns in gene expression, biochemical features and morphologic assessments that may offer meaningful insight into this relationship.
针对卵丘细胞的生物学过程和卵母细胞质量的测量方法进行了一项系统综述。初步检索得到18549篇文章，经过筛选，本综述纳入了65项研究。对提取的数据进行了描述性综合分析。文章根据所涉及的卵丘细胞的生物学特征进行分类：基因表达、生化活性和形态特征。还对涉及卵丘细胞功能与妇科疾病及其对卵母细胞质量影响的手稿进行了综述。六项研究的结果表明，与细胞外基质发育相关的通路（HAS2、VCAN）表达增加与卵母细胞质量改善之间存在关联。六篇手稿报道了与卵丘细胞扩张相关的基因（GREM1、PTGS2）与卵母细胞质量改善有关，六篇文章指出了凋亡通路调节因子（BCL2、BIRC5、MDM2、PGAM5）的表达模式。十二项研究检测了卵丘细胞的生化特征，包括卵丘线粒体膜电阻、呼吸测量指数和乙酰辅酶A水平，这些都与卵母细胞发育有关。五项研究评估了卵丘细胞的形态学，证明线粒体大小、端粒长度以及卵丘密度/分散度与卵母细胞质量之间存在关系。二十二篇文章评估了多囊卵巢综合征（PCOS）和子宫内膜异位症等疾病以及产妇年龄增加（无论卵巢储备是否降低）时卵丘细胞功能及其与卵母细胞质量的关系。这项全面的系统综述整合了研究卵丘细胞与卵母细胞质量之间关系的研究结果，确定了基因表达、生化特征和形态学评估中的模式，这些模式可能为这种关系提供有意义的见解。
