University of Münster, Institute of Pharmaceutical Biology and Phytochemistry, Münster, Germany.
Glycobiology. 2012 Nov;22(11):1424-39. doi: 10.1093/glycob/cws107. Epub 2012 Jul 6.
Water-soluble high molecular weight compounds were isolated in yields of 21-22% from the oleogum of Boswellia serrata and B. carteri. Using anion exchange chromatography and gel permeation chromatography, different proteoglycans were purified and characterized, leading to four principally different groups: (i) Hyp-/Ser-rich extensins with O-glycosidic attached arabinan side chains; (ii) Modified extensins, with arabinogalactosylated side chains containing GlA and 4-O-Me-GlcA; (iii) Glycoproteins with N-glycosidic side chains containing higher amounts of Fuc, Man and GluNH(2,) featuring a 200 kD metalloproteinase that has been de novo sequenced and is described for the first time; (iv) Type II arabinogalactans-proteins. Significant differences between the gums from the two species were observed in the protein content (6% vs 22%), offering the possibility of a quick differentiation of gums from both species for analytical quality control. The data also offer an insight into the plant response towards wound-closing by the formation of extensin and AGP-containing gum.
从乳香树（Boswellia serrata）和卡氏乳香树（B. carteri）的油胶中分别以 21-22%的产率分离得到水溶性高分子量化合物。采用阴离子交换色谱和凝胶渗透色谱法，对不同的糖蛋白进行了纯化和表征，结果表明存在四种主要不同的糖蛋白：（i）富含羟脯氨酸/丝氨酸的伸展蛋白，具有 O-糖基化连接的阿拉伯聚糖侧链；（ii）阿拉伯半乳聚糖化的修饰伸展蛋白，其侧链含有 GlA 和 4-O-Me-GlcA；（iii）具有 N-糖基化侧链的糖蛋白，含有较多的 Fuc、Man 和 GluNH2，其特征是具有 200kD 的金属蛋白酶，该蛋白酶已从头测序并首次描述；（iv）Ⅱ型阿拉伯半乳聚糖-蛋白质。两种胶中观察到蛋白质含量（6%对 22%）存在显著差异，这为两种胶的快速鉴别提供了可能，可用于分析质量控制。该数据还为植物对伤口闭合的反应提供了一些线索，即通过形成伸展蛋白和富含 AGP 的胶来实现。
