Institute of Endocrinology and Nutrition, Medicine School and Unit of Investigation, Hospital Rio Hortega, University of Valladolid, Valladolid, Spain RD056/0001 3RETICEF.
Eur Rev Med Pharmacol Sci. 2012 May;16(5):610-6.
The aim of our study was to investigate the interaction of tryptophan-to-arginine (Trp64Arg) missense mutation in the beta3 adrenoreceptor (Beta3AR) with polymorphism in the UCP3 promotor (-55C->T) on insulin resistance in obese patients.
A population of 212 obese patients was analyzed. A bipolar electrical bioimpedance, a biochemical analysis and concentrations of adipocytokines were assessed.
One hundred and sixty-two patients (76.4%) had the genotype Trp64/Trp64 (wild type group) and 50 patients Trp64/Arg64 (23.6%) (mutant type group). One hundred and seventy five (87.2%) had the genotype -55CC (wild type group) and 27 patients (22.8%) -55CT (mutant type group). Five patients (2.4%) had both polymorphisms Trp64/Arg64 and -55CT. Patients with one or both mutant genotypes had higher BMI, weight, fat mass, systolic blood pressure and waist circumference than wild type patients. Patients with 55CT or 55CT and Trp64Arg genotype had higher BMI, weight, fat mass, waist circumference, waist to hip ratio glucose, insulin, triglycerides and HOMA than wild type or Trp64Arg mutation.
Higher concentrations of insulin, HOMA, triglycerides, glucose, BMI, weight, fat mass, waist to hip ratio and waist circumference were observed in patients with -55CT genotype alone or -55CT plus Trp64Arg genotypes than in patients without mutation or only Trp64Arg mutation.
我们的研究目的是探究肥胖患者中β3 肾上腺素能受体（Beta3AR）的色氨酸到精氨酸（Trp64Arg）错义突变与 UCP3 启动子（-55C->T）多态性的相互作用对胰岛素抵抗的影响。
分析了 212 名肥胖患者。采用双极电生物阻抗法、生化分析和细胞因子浓度评估。
162 名患者（76.4%）携带 Trp64/Trp64（野生型）基因型，50 名患者携带 Trp64/Arg64（突变型）基因型。175 名患者（87.2%）携带-55CC（野生型）基因型，27 名患者（22.8%）携带-55CT（突变型）基因型。5 名患者（2.4%）同时携带 Trp64/Arg64 和-55CT 两种突变基因型。携带一种或两种突变基因型的患者 BMI、体重、体脂、收缩压和腰围均高于野生型患者。携带-55CT 或-55CT 和 Trp64Arg 基因型的患者 BMI、体重、体脂、腰围、腰臀比、血糖、胰岛素、甘油三酯和 HOMA 均高于野生型或 Trp64Arg 突变型患者。
与无突变或仅 Trp64Arg 突变的患者相比，携带-55CT 基因型或-55CT 加 Trp64Arg 基因型的患者胰岛素、HOMA、甘油三酯、血糖、BMI、体重、体脂、腰臀比和腰围的浓度更高。
