Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Dicle University, Diyarbakir, Turkey.
Eur Rev Med Pharmacol Sci. 2012 May;16(5):679-86.
The aim of this study was to compare culture-expanded, bone marrow-derived mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) loaded to biphasic calcium phosphate (BCP) bone ceramic in the repair of rat calvarial bone.
Critical-size (7 mm dia.) calvarial defects were prepared in the frontal-parietal bones of 90 adult female Sprague-Dawley rats. Rats were randomly divided into 5 groups, according to defect filling, as follows: Group I (n = 21), BCP; Group II (n = 21), BCP+PRP; Group III (n = 21), BCP+MSC; Group IV (n = 21), BCP+PRP+MSC; Group V (n = 6) (control), no treatment. Animals were sacrificed at 2, 8 and 12 weeks postsurgery and bone regeneration was evaluated both histologically and immunohistochemically.
Statistically significant differences were observed in bone osteoblastic activity in calvarial defects among the groups (p < 0.05). PRP and MSC used in combination with BCP as a defect filling resulted in greater osteoblastic bone formation activity when compared to the use of BCP alone.
The combination of mesenchymal stem cells, platelet rich plasma and synthetic bone substitute was found to be more effective in inducing new bone formation (osteogenesis) than the use of platelet rich plasma combined with synthetic bone substitute and the use of synthetic bone substitute alone.
本研究旨在比较体外培养的骨髓间充质干细胞（MSCs）和负载富血小板血浆（PRP）的双相磷酸钙（BCP）骨陶瓷在修复大鼠颅骨中的作用。
在 90 只成年雌性 Sprague-Dawley 大鼠的额顶骨上制备临界尺寸（7mm 直径）的颅骨缺损。根据缺损填充情况，将大鼠随机分为 5 组，如下：第 I 组（n = 21），BCP；第 II 组（n = 21），BCP+PRP；第 III 组（n = 21），BCP+MSC；第 IV 组（n = 21），BCP+PRP+MSC；第 V 组（n = 6）（对照组），不做任何处理。术后 2、8 和 12 周处死动物，通过组织学和免疫组织化学评估骨再生情况。
各组颅骨缺损骨成骨活性存在统计学差异（p < 0.05）。与单独使用 BCP 相比，PRP 和 MSC 与 BCP 联合作为缺损填充物可导致更强的成骨活性。
与单独使用富血小板血浆联合合成骨替代物以及单独使用合成骨替代物相比，间充质干细胞、富血小板血浆和合成骨替代物的联合使用在诱导新骨形成（成骨）方面更有效。
