Department of Chemistry and Biochemistry, Edison Biotechnology Institute, Ohio University, Athens, OH, USA.
Exp Dermatol. 2012 Aug;21(8):640-2. doi: 10.1111/j.1600-0625.2012.01547.x.
Upon UVB irradiation, an alternation of major lipid raft components can lead to the recruitment/activation of rafts-associated proteins and initiation of downstream apoptotic signalling pathways. We used two-dimensional gel electrophoresis (2-DE) to identify potential regulators of UVB-induced apoptosis and mass spectrometry fingerprint analysis to identify proteins that are altered in the rafts after UVB irradiation. Our data show that levels of several proteins, including prohibitin (PHB), were changed in lipid rafts after UVB irradiation. We also demonstrate that while total PHB expression was not changed, the protein was enriched in lipid rafts after UVB irradiation. Reduced expression of PHB using siRNA knockdown resulted in an increase in cellular apoptosis after UVB irradiation. Based on these results, we propose that PHB protects keratinocytes from UVB-induced apoptosis.
在 UVB 照射下，主要脂质筏成分的交替变化可能导致筏相关蛋白的募集/激活，并启动下游凋亡信号通路。我们使用二维凝胶电泳（2-DE）来鉴定潜在的 UVB 诱导凋亡调节剂，并通过质谱指纹分析鉴定 UVB 照射后在筏中发生改变的蛋白质。我们的数据表明，包括 prohibitin（PHB）在内的几种蛋白质的水平在 UVB 照射后在脂筏中发生改变。我们还证明，虽然总 PHB 表达没有改变，但该蛋白在 UVB 照射后在脂筏中富集。用 siRNA 敲低降低 PHB 的表达会导致 UVB 照射后细胞凋亡增加。基于这些结果，我们提出 PHB 可保护角质形成细胞免受 UVB 诱导的凋亡。
