Dermatological Specialized Center "DERMAL" NZOZ in Białystok, Nowy Swiat 17/5, 15-453 Białystok, Poland.
Laboratory of Bioanalysis, Faculty of Chemistry, University in Białystok, Ciołkowskiego 1K, 15-245 Białystok, Poland.
Int J Mol Sci. 2023 Aug 4;24(15):12424. doi: 10.3390/ijms241512424.
The action of UVA radiation (both that derived from solar radiation and that used in the treatment of skin diseases) modifies the function and composition of keratinocyte membranes. Therefore, this study aimed to assess the effects of phytocannabinoids (CBD and CBG), used singly and in combination, on the contents of phospholipids, ceramides, lipid rafts and sialic acid in keratinocyte membranes exposed to UVA radiation, together with their structure and functionality. The phytocannabinoids, especially in combination (CBD+CBG), partially prevented increased levels of phosphatidylinositols and sialic acid from occurring and sphingomyelinase activity after the UVA exposure of keratinocytes. This was accompanied by a reduction in the formation of lipid rafts and malondialdehyde, which correlated with the parameters responsible for the integrity and functionality of the keratinocyte membrane (membrane fluidity and permeability and the activity of transmembrane transporters), compared to UVA-irradiated cells. This suggests that the simultaneous use of two phytocannabinoids may have a protective effect on healthy cells, without significantly reducing the therapeutic effect of UV radiation used to treat skin diseases such as psoriasis.
UVA 辐射（包括源自太阳辐射和用于治疗皮肤病的辐射）的作用会改变角质形成细胞膜的功能和组成。因此，本研究旨在评估单独和联合使用植物大麻素（CBD 和 CBG）对暴露于 UVA 辐射的角质形成细胞膜中磷脂、神经酰胺、脂筏和唾液酸含量的影响，以及它们的结构和功能。植物大麻素，尤其是联合使用时（CBD+CBG），部分预防了 UVA 辐射后角质形成细胞中磷脂酰肌醇和唾液酸水平的升高以及神经鞘磷脂酶的活性。这伴随着脂质筏和丙二醛的形成减少，与角质形成细胞膜完整性和功能相关的参数（膜流动性和通透性以及跨膜转运蛋白的活性）相关，与 UVA 辐射的细胞相比。这表明，两种植物大麻素的同时使用可能对健康细胞具有保护作用，而不会显著降低用于治疗银屑病等皮肤病的 UV 辐射的治疗效果。
