Knappy Christopher S, Chong James P J, Keely Brendan J
Department of Chemistry, University of York, York, United Kingdom.
J Am Soc Mass Spectrom. 2009 Jan;20(1):51-9. doi: 10.1016/j.jasms.2008.09.015. Epub 2008 Sep 23.
Atmospheric pressure chemical ionization liquid chromatography-tandem mass spectrometry (APCI LC-MS/MS) of tetraether lipid cores of archaeal origin reveals distinct dissociation pathways for three classes of core lipid extracted from Methanobacter thermautotrophicus. Within these classes, two isobaric tetraether lipids, one a scarcely reported lipid constituent of M. thermautotrophicus and the other an artefact formed during extraction from cultured cells, were identified and distinguished via their MS(2) spectra. APCI LC-MS/MS discriminates different tetraether core lipid types and isobaric species and reveals the mass of the constituent biphytanyl chains within the tetraether cores, albeit without full elucidation of their structures. Furthermore, the method allows direct estimation of the relative proportions of tetraether core lipids from chromatographic peak area measurement, allowing rapid profiling of these compounds in microbiological and environmental extracts.
对源自古菌的四醚脂质核心进行大气压化学电离液相色谱-串联质谱分析（APCI LC-MS/MS），揭示了从嗜热自养甲烷杆菌中提取的三类核心脂质的不同解离途径。在这些类别中，通过二级质谱（MS(2)）谱图鉴定并区分了两种等压四醚脂质，一种是嗜热自养甲烷杆菌中鲜有报道的脂质成分，另一种是从培养细胞中提取过程中形成的人工产物。APCI LC-MS/MS能够区分不同的四醚核心脂质类型和等压物种，并揭示四醚核心内组成性双植烷链的质量，尽管无法完全阐明其结构。此外，该方法可通过色谱峰面积测量直接估算四醚核心脂质的相对比例，从而快速分析微生物和环境提取物中的这些化合物。
